Development of the process for extracting and isolating hesperidin from Chenbi to establish as a reference standard

Hesperidin is the main active ingredient in the flavonoid group, accounting for about 3 % of Chenbi. According to studies, hesperidin has anti-oxidant, analgesic, and anti-inflammatory effects on the nervous system as well as enhancing the body's resistance. Chenbi is a simple medicine, easy to obtain, and uses medicinal material for the potential extraction and isolation of Hesperin with high yield. The study developed and validated the process of determining hesperidin on medicinal samples and raw materials by HPLC. The study was successful in making the active ingredient hesperidin with a purity > 95 % (HPLC) sufficient as a condition to be used as a referenced material. Extraction and isolation process using 70 % ethanol extraction solvent with extraction method is reflux heating then cleaning with activated carbon and purified on chromatographic equipment to obtain high recovery efficiency, simple and economical process.