DPPH法测定5个枣品种在不同时期的自由基清除能力

Z. A. Mahar, G. Q. Shar, B. Lal, Z. Solangi
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引用次数: 0

摘要

从Khairpur Mir地区收集了枣果实样品,并检测了其抗氧化活性,为此,采用DPPH法在不同的稀释剂中制备了提取物,如甲醇+水(50:50),甲醇和水。实验结果表明,所有提取物均具有较强的抗自由基能力。其中溶液对自由基的抑制能力较强。结果表明,Gajar Wari的活性较强,在甲醇中IC50值最低(20.022µg/mL),而其他品种在溶液末阶段的IC50值最低(18.33µg/mL), Aseel品种的活性也较低,在第二阶段的甲醇中具有较强的抗自由基能力。
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Free Radical Scavenging Potency of Five Date Varieties at Different Stages by Using DPPH Assay
Samples of date fruit was collected from Khairpur Mir’s district and examined for antioxidant activity, for this purpose extract was prepared in different diluents like Methanol+Water (50:50), methanol and water by using DPPH assay. It was observed from data that all the extract have impressive antioxidant ability against free radicals. Among them solution have more ability to ramp the free radicals. From data, it was concluded that Gajar Wari is more active as it possess lowest IC50 value (20.022 µg/mL in Methanol) then rest of other date varieties at final stage in solution as well as Aseel variety also possesses lowest IC50 value (18.33 µg/mL), there for have more ability against free radicals in methanol at second stage.
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