微流式细胞仪的CAD研究及其在细菌检测中的应用

S.S. Lee, S. Izuo, K. Inatomi
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引用次数: 2

摘要

我们设计并制作了一种用于细菌检测的微流式细胞仪。在本文中,我们已经做了CAD研究,以获得该装置的最佳工作条件。在模拟中,我们研究了样品和护套流的流量比,以确定在期望位置的流体动力聚焦和在检测位置适当的样品流宽度。因此,我们可以获得足够的样品和护套流的流量比,我们的设备在1:5附近。根据模拟结果进行了细菌计数实验,并在预期位置和适当的样品流量下实现了流体动力聚焦。
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A CAD study on micro flow cytometer and its application to bacteria detection
We have designed and fabricated a micro flow cytometer for application to bacteria detection. In this paper, we present a CAD study which has been done to obtain optimal working conditions of the device. In the simulation, we have investigated flow rate ratios of sample and sheath flow to confirm hydrodynamic focusing at a desired position and proper sample stream width at the detection position. As a result, we could obtain adequate flow rate ratios of sample and sheath flow, which is in the vicinity of 1:5 for our device. A bacteria enumeration experiment has also been carried out, based on the simulation results, and we have achieved hydrodynamic focusing at the expected position and proper sample flow rate.
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