亚慢性铅暴露大鼠的血液代谢指纹图谱

A. Chemezov, M. Sutunkova, Ilzira Minigalieva, S. Klinova, I. Ryabova
{"title":"亚慢性铅暴露大鼠的血液代谢指纹图谱","authors":"A. Chemezov, M. Sutunkova, Ilzira Minigalieva, S. Klinova, I. Ryabova","doi":"10.18332/pht/144486","DOIUrl":null,"url":null,"abstract":"INTRODUCTION Environmental pollution with heavy metals is vital issue in the present time. Lead, as one of the environmental contaminators, deserves attention due to its applicability in human activity and its xenobiotic character. METHODS In this study we used an untargeted metabolomic approach – the metabolomic fingerprinting method. Lead acetate was intraperitoneally injected to rats within the framework of a subchronical experiment and blood samples were analyzed by UHPLC-MS. Metabolite expression was qualitatively compared between treated and control groups. For substances with a significant content difference, tandem CID spectra for structure elucidation were obtained using an exact mass search by generating a list of potential formulas, by a characteristic fragments database search, using in silico mass spectra prediction, and by looking for a specific fragmentation rearrangement. RESULTS Untargeted metabolomic analysis shows 37 metabolites with significant changes in expression in comparing the control and exposure group. Annotation by the fragment spectra analysis was carried out for 8 metabolites. These include epoxy-derivative of linoleic acid, hydroxyand oxoacylcarnitines of long-chain fatty acids, 15-hydroxyeicosatetraenoic acid, acylcarnitine of middlechain fatty acid and lysophosphatidylserine derivative. CONCLUSIONS Literature analysis allows to establish known functions of annotated metabolites. Connections to manifestations of lead poisoning were established. Specifically, hydroxyand oxoacylcarnitines are intermediates in the beta-oxidation process of fatty acids – increased concentration of these derivatives may be a sign of disturbances in this process under the influence of leadinduced oxidative stress. Epoxyand hydroxyderivatives of polyunsaturated fatty acids, which demonstrate elevated concentration levels possess regulatory activity (vasodynamic, antiapoptotic, and antioxidative action) on the one hand, and presumably are biomarkers of oxidative stress. Elevated levels of lysophosphatidylserine may be evidence of apoptotic process intensification.","PeriodicalId":20841,"journal":{"name":"Public Health Toxicology","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2021-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Blood metabolic fingerprinting of rats, subjected to subchronic lead exposure\",\"authors\":\"A. Chemezov, M. Sutunkova, Ilzira Minigalieva, S. Klinova, I. Ryabova\",\"doi\":\"10.18332/pht/144486\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"INTRODUCTION Environmental pollution with heavy metals is vital issue in the present time. Lead, as one of the environmental contaminators, deserves attention due to its applicability in human activity and its xenobiotic character. METHODS In this study we used an untargeted metabolomic approach – the metabolomic fingerprinting method. Lead acetate was intraperitoneally injected to rats within the framework of a subchronical experiment and blood samples were analyzed by UHPLC-MS. Metabolite expression was qualitatively compared between treated and control groups. For substances with a significant content difference, tandem CID spectra for structure elucidation were obtained using an exact mass search by generating a list of potential formulas, by a characteristic fragments database search, using in silico mass spectra prediction, and by looking for a specific fragmentation rearrangement. RESULTS Untargeted metabolomic analysis shows 37 metabolites with significant changes in expression in comparing the control and exposure group. Annotation by the fragment spectra analysis was carried out for 8 metabolites. These include epoxy-derivative of linoleic acid, hydroxyand oxoacylcarnitines of long-chain fatty acids, 15-hydroxyeicosatetraenoic acid, acylcarnitine of middlechain fatty acid and lysophosphatidylserine derivative. CONCLUSIONS Literature analysis allows to establish known functions of annotated metabolites. Connections to manifestations of lead poisoning were established. Specifically, hydroxyand oxoacylcarnitines are intermediates in the beta-oxidation process of fatty acids – increased concentration of these derivatives may be a sign of disturbances in this process under the influence of leadinduced oxidative stress. Epoxyand hydroxyderivatives of polyunsaturated fatty acids, which demonstrate elevated concentration levels possess regulatory activity (vasodynamic, antiapoptotic, and antioxidative action) on the one hand, and presumably are biomarkers of oxidative stress. Elevated levels of lysophosphatidylserine may be evidence of apoptotic process intensification.\",\"PeriodicalId\":20841,\"journal\":{\"name\":\"Public Health Toxicology\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2021-12-22\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Public Health Toxicology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.18332/pht/144486\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Public Health Toxicology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.18332/pht/144486","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

摘要

重金属环境污染是当今社会面临的重要问题。铅作为一种环境污染物,因其在人类活动中的适用性和外生特性而备受关注。方法在本研究中,我们使用了一种非靶向代谢组学方法-代谢组学指纹图谱法。在亚慢性实验框架内,大鼠腹腔注射醋酸铅,并用UHPLC-MS分析血液样本。对实验组和对照组的代谢物表达进行定性比较。对于具有显著含量差异的物质,采用精确的质量搜索(生成潜在公式列表)、特征片段数据库搜索、硅质谱预测和寻找特定片段重排来获得用于结构解析的串联CID光谱。结果非靶向代谢组学分析显示,与对照组和暴露组相比,37种代谢物的表达有显著变化。对8种代谢物进行了片段谱分析注释。其中包括亚油酸的环氧衍生物、长链脂肪酸的羟基和氧酰基肉碱、15-羟基二十碳四烯酸、中链脂肪酸的酰基肉碱和溶血磷脂酰丝氨酸衍生物。结论:通过文献分析,可以确定已注释代谢物的已知功能。建立了与铅中毒表现的联系。具体来说,羟基和氧酰基肉碱是脂肪酸β -氧化过程中的中间体-这些衍生物浓度的增加可能是铅诱导氧化应激影响下该过程紊乱的标志。多不饱和脂肪酸的环氧和羟基衍生物,其浓度水平升高,一方面具有调节活性(血管动力学、抗凋亡和抗氧化作用),可能是氧化应激的生物标志物。溶血磷脂酰丝氨酸水平升高可能是细胞凋亡过程增强的证据。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
Blood metabolic fingerprinting of rats, subjected to subchronic lead exposure
INTRODUCTION Environmental pollution with heavy metals is vital issue in the present time. Lead, as one of the environmental contaminators, deserves attention due to its applicability in human activity and its xenobiotic character. METHODS In this study we used an untargeted metabolomic approach – the metabolomic fingerprinting method. Lead acetate was intraperitoneally injected to rats within the framework of a subchronical experiment and blood samples were analyzed by UHPLC-MS. Metabolite expression was qualitatively compared between treated and control groups. For substances with a significant content difference, tandem CID spectra for structure elucidation were obtained using an exact mass search by generating a list of potential formulas, by a characteristic fragments database search, using in silico mass spectra prediction, and by looking for a specific fragmentation rearrangement. RESULTS Untargeted metabolomic analysis shows 37 metabolites with significant changes in expression in comparing the control and exposure group. Annotation by the fragment spectra analysis was carried out for 8 metabolites. These include epoxy-derivative of linoleic acid, hydroxyand oxoacylcarnitines of long-chain fatty acids, 15-hydroxyeicosatetraenoic acid, acylcarnitine of middlechain fatty acid and lysophosphatidylserine derivative. CONCLUSIONS Literature analysis allows to establish known functions of annotated metabolites. Connections to manifestations of lead poisoning were established. Specifically, hydroxyand oxoacylcarnitines are intermediates in the beta-oxidation process of fatty acids – increased concentration of these derivatives may be a sign of disturbances in this process under the influence of leadinduced oxidative stress. Epoxyand hydroxyderivatives of polyunsaturated fatty acids, which demonstrate elevated concentration levels possess regulatory activity (vasodynamic, antiapoptotic, and antioxidative action) on the one hand, and presumably are biomarkers of oxidative stress. Elevated levels of lysophosphatidylserine may be evidence of apoptotic process intensification.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
Prevention and reversal of chronic diseases: A Protocol Public health and public law issues for the toxicological risk assessment of chemical mixtures Blood metabolic fingerprinting of rats, subjected to subchronic lead exposure Utilization of insecticides treated mosquito bed nets among pregnant women in Kassena-Nankana East municipality in the upper east region of Ghana Unveiling the effect of telomeres length in cancers development
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1