Modelling and Tissue Engineering of Three Layers of Calvarial Bone as a Biomimetic Scaffold

In this Study, a New Zealand Rabbit Parietal Bone Was Cross-Sectioned, and Parameters such as Entire Thickness and the Thicknesses of the Compact and Spongy Tables Were Morphometrically Measured by Imagej Software. the Pore Size of the Cancellous Table Was Also Analysed, and a Calvarial Bone Model Was Created. Based upon a Natural Model for Bone Repair, a Nano-Structured Scaffold Was Designed Using Bioglass and Gelatin (BG) as its Main Components. the Scaffold Was Prepared Using Layer Solvent Casting Combined with Freeze-Drying, Layering Techniques, and other Commonly Used Techniques. the Fabricated BG Scaffolds Were Made with Different Percentages of Nanoparticles, and the 10% and 30% Constructions Were Found to Be Respectively Similar to Compact and Spongy Bone. we Fabricated Three Lamellar Scaffolds with Two Compact Layers on the outside and One Spongy Layer in the Middle to Mimic the Composition and Structure of Natural Bone. the Chemical, Physical, and Biological Tests (including Cell Seeding on Scaffold and MTT Assay) that Evaluated this Scaffold Examined its Capacity to Promote Bone Repair. Fabricated Scaffolds Implanted in Rabbit Calvaria and Evaluated the Bone Repair by X-Ray. this Mimetic BG Scaffold Could Be an Excellent Candidate for a Synthetic Calvarial Bone Graft.