E. Shamilov, A. Abdullayev, V. E. Shamilli, I. Azizov
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Determination of chlorophylls and carotenoids was carried out according to Shlyk. Results. g-irradiation at doses of 50, 100 and 200 Gy has a significant effect on the content of green pigments and carotenoids in wheat seedlings. Under the action of g-irradiation, the content of chlorophyll decreases more than carotenoids. With an increase in the dose of radiation, a slight increase in the content of carotenoids is noted. In all variants, chromosomal abnormalities were found: the formation of fragments in metaphase and anaphase, bridges in anaphase, chromosome delays, uneven division of chromosomes. Conclusions. For the first time, it was found that the Zn (II) complex of hypoxanthine-9-riboside at the indicated concentrations significantly reduces the damaging effect of γ-irradiation, helps to eliminate abnormalities in mitotic division in wheat root hair cells.","PeriodicalId":12181,"journal":{"name":"Faktori eksperimental'noi evolucii organizmiv","volume":"5 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Protective effect of zinc complex with hypoxanthine-9-riboside on wheat seedlings grown from gamma-irradiated seeds\",\"authors\":\"E. Shamilov, A. Abdullayev, V. E. Shamilli, I. Azizov\",\"doi\":\"10.7124/feeo.v31.1504\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Aim. The aim of the research was to obtain the zinc complex hypoxanthine-9-riboside and to study its effect during γ-irradiation on the biosynthesis of chlorophylls, carotenoids and on the release of chromosome aberrations in anaphase root hair cells in wheat seedlings. Methods. The zinc complex was obtained by direct interaction of zinc chloride – ZnCl2 with hypoxanthine-9-riboside. X-ray phase analysis and thermogravimetric measurements of the obtained complex were carried out. Before irradiation, seeds of durum wheat Triticum durum L. from a 60Co source were treated with a zinc complex with hypoxanthine-9-riboside at concentrations of 0.1; 0.01; 0.001%. Structural changes in chromosomes were determined in the initial and final stages of anaphase. Determination of chlorophylls and carotenoids was carried out according to Shlyk. Results. g-irradiation at doses of 50, 100 and 200 Gy has a significant effect on the content of green pigments and carotenoids in wheat seedlings. Under the action of g-irradiation, the content of chlorophyll decreases more than carotenoids. With an increase in the dose of radiation, a slight increase in the content of carotenoids is noted. In all variants, chromosomal abnormalities were found: the formation of fragments in metaphase and anaphase, bridges in anaphase, chromosome delays, uneven division of chromosomes. Conclusions. For the first time, it was found that the Zn (II) complex of hypoxanthine-9-riboside at the indicated concentrations significantly reduces the damaging effect of γ-irradiation, helps to eliminate abnormalities in mitotic division in wheat root hair cells.\",\"PeriodicalId\":12181,\"journal\":{\"name\":\"Faktori eksperimental'noi evolucii organizmiv\",\"volume\":\"5 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2022-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Faktori eksperimental'noi evolucii organizmiv\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.7124/feeo.v31.1504\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Faktori eksperimental'noi evolucii organizmiv","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.7124/feeo.v31.1504","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
的目标。本研究的目的是获得锌络合物次黄嘌呤-9-核苷,并研究其在γ辐照下对小麦幼苗后期根毛细胞叶绿素、类胡萝卜素生物合成及染色体畸变释放的影响。方法。氯化锌- ZnCl2与次黄嘌呤-9-核苷直接反应得到锌配合物。对所得配合物进行了x射线物相分析和热重测量。辐照前,用浓度为0.1的次黄嘌呤-9-核苷锌络合物处理来自60Co源的硬粒小麦(Triticum durum L.)种子;0.01;0.001%。染色体的结构变化是在后期的初始和最后阶段确定的。按Shlyk法测定叶绿素和类胡萝卜素。结果。50、100和200 Gy剂量的g辐照对小麦幼苗中绿色色素和类胡萝卜素含量有显著影响。在g辐照作用下,叶绿素含量下降幅度大于类胡萝卜素。随着辐射剂量的增加,类胡萝卜素的含量略有增加。在所有变异中均发现染色体异常:中期和后期形成片段,后期出现桥接,染色体延迟,染色体分裂不均匀。结论。首次发现,在指定浓度下,次黄嘌呤-9-核苷的Zn (II)配合物可显著降低γ辐照的损伤作用,有助于消除小麦根毛细胞有丝分裂的异常。
Protective effect of zinc complex with hypoxanthine-9-riboside on wheat seedlings grown from gamma-irradiated seeds
Aim. The aim of the research was to obtain the zinc complex hypoxanthine-9-riboside and to study its effect during γ-irradiation on the biosynthesis of chlorophylls, carotenoids and on the release of chromosome aberrations in anaphase root hair cells in wheat seedlings. Methods. The zinc complex was obtained by direct interaction of zinc chloride – ZnCl2 with hypoxanthine-9-riboside. X-ray phase analysis and thermogravimetric measurements of the obtained complex were carried out. Before irradiation, seeds of durum wheat Triticum durum L. from a 60Co source were treated with a zinc complex with hypoxanthine-9-riboside at concentrations of 0.1; 0.01; 0.001%. Structural changes in chromosomes were determined in the initial and final stages of anaphase. Determination of chlorophylls and carotenoids was carried out according to Shlyk. Results. g-irradiation at doses of 50, 100 and 200 Gy has a significant effect on the content of green pigments and carotenoids in wheat seedlings. Under the action of g-irradiation, the content of chlorophyll decreases more than carotenoids. With an increase in the dose of radiation, a slight increase in the content of carotenoids is noted. In all variants, chromosomal abnormalities were found: the formation of fragments in metaphase and anaphase, bridges in anaphase, chromosome delays, uneven division of chromosomes. Conclusions. For the first time, it was found that the Zn (II) complex of hypoxanthine-9-riboside at the indicated concentrations significantly reduces the damaging effect of γ-irradiation, helps to eliminate abnormalities in mitotic division in wheat root hair cells.