黑曲霉发酵菠萝废弃物生产柠檬酸的研究

A. Ayeni, M. Daramola, O. Taiwo, Omowonuola I. Olanrewaju, D. Oyekunle, P. Sekoai, F. Elehinafe
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引用次数: 20

摘要

柠檬酸除了用作清洁剂外,在化学、制药和食品工业中也有各种各样的应用。生物技术发酵过程是满足这种有用商品需求的最简单方法之一。以菠萝渣为原料,对黑曲霉发酵生产柠檬酸进行了研究。STATISTICA 8发布7 (Statsoft, Inc.)采用响应面法中心复合设计(CCD),采用统计软件进行实验设计、评价和工艺优化。设计的操作参数下限为温度(25-35℃)、发酵时间(35-96 h)、pH(3-6)、甲醇浓度(1-7%)和葡萄糖(15-85 g/L)。对CCD线路进行了27次重复实验。结果表明:葡萄糖浓度为38 g/L,甲醇浓度为3% (v/v),发酵时间为50 h, pH为4.3,温度为30℃,柠檬酸产率为15.51 g/L。采用单因素方差分析评估模型的统计学显著性。统计模型验证的预测响应值与实验数据具有较好的相关性。
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Production of Citric Acid from the Fermentation of Pineapple Waste by Aspergillus niger
Citric acid, aside its uses as a cleaning agent, has varied applications in the chemical, pharmaceutical, and food industries. A biotechnological fermentation process is one of the easiest ways to satisfy the demands for this useful commodity.The fermentation of pineapple waste byAspergillus nigerfor the production of citric acid was investigated in this study. STATISTICA 8 release 7 (Statsoft, Inc. USA) statistical software was used for the design of experiments, evaluation, and optimization of the process using the central composite design (CCD), a response surface methodology approach. Lower-upper limits of the design for the operating parameters were temperature (25-35oC), fermentation time (35-96 h), pH (3-6), methanol concentration (1-7%) and glucose (15-85 g/L). Twenty-seven duplicated experimental runs were generated for the CCD route.The optimal operating conditions were validated at 38 g/L of glucose concentration, 3% (v/v) of methanol, 50 h of fermentation time, pH of 4.3 and temperature of 30oC which yielded15.51 g/L citric acid. The statistical significance of the model was evaluated using a one-way analysis of variance. The validated predicted response values obtained from the statistical model showed close relationships with the experimental data.
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