C. Ikese, S. T. Ubwa, P. A. Adie, S. Audu, Godwin Ejeke
{"title":"一种配方婴儿食品对Wistar大鼠某些生物学指标的性能评价","authors":"C. Ikese, S. T. Ubwa, P. A. Adie, S. Audu, Godwin Ejeke","doi":"10.2478/auoc-2019-0021","DOIUrl":null,"url":null,"abstract":"Abstract Performance evaluation of a formulated infant food on some biological indices in Wistar rats was investigated. For rats fed separately with normal rat feed, formulated infant food and proprietary food, serum total protein and albumin were determined by colorimetric method, red blood cell (RBC) and differential white blood cell (WBC) counts were determined with a haemocytometer, packed cell volume (PCV) was by micro-haematocrit, haemoglobin (HGB) was determined using cyanomethaemoglobin method while mean cell volume (MCV) and mean cell haemoglobin (MCH) were calculated. The results obtained showed that, for Wistar rats fed with normal rat feed, the baseline values prior to the commencement of feeding trial for serum total protein, albumin, body weight, PCV, WBC, platelet count (PLT), RBC, HGB, lymphocyte count (LYM), MCH, MCV, neutrophil (NEU), and eosinophil (EOS) were 82.9 g/dL, 44.75 g/dL, 142.80g, 41.14%, 7.60×103 μL−1, 404.85×103 μL−1, 5.68×106 μL−1, 10.80 g/dL, 50.28 %, 18.42 pg, 59.28 fL, 45.57 %, and 2.57 % respectively. After the feeding trial, the serum total protein, albumin, body weight, PCV, WBC, PLT, RBC, HGB, LYM, MCH, MCV, NEU, and EOS in Wistar rats fed with the formulated infant food (the experimental group) were 79.6 g/dL, 50.65 g/dL, 169.18g, 40.14%, 5.77 ×103 μL−1, 309.85 ×103 μL−1, 6.85×106 μL−1, 12.45 g/dL, 52.85 %, 18.24 pg, 58.64 fL, 42.00 %, and 2.14% respectively and these were in most cases, either greater or comparable with their corresponding mean values of 69.1 g/dL, 44.40 g/dL, 177.97 g, 30.24%, 4.18×103 μL−1, 188.14×103 μL−1, 5.29×106 μL−1, 10.74 g/dL, 53.14 %, 20.37 pg, 57.61 fL, 38.85 %, and 4.57 % respectively in Wistar rats fed with the proprietary infant formula (the control group). This shows that the potential infant food has a more positive effect on most of the biological indices assessed than the proprietary infant food or a comparable effect at the least.","PeriodicalId":19641,"journal":{"name":"Ovidius University Annals of Chemistry","volume":null,"pages":null},"PeriodicalIF":1.0000,"publicationDate":"2019-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Performance evaluation of a formulated infant food on some biological indices in Wistar rats\",\"authors\":\"C. Ikese, S. T. Ubwa, P. A. Adie, S. Audu, Godwin Ejeke\",\"doi\":\"10.2478/auoc-2019-0021\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Abstract Performance evaluation of a formulated infant food on some biological indices in Wistar rats was investigated. For rats fed separately with normal rat feed, formulated infant food and proprietary food, serum total protein and albumin were determined by colorimetric method, red blood cell (RBC) and differential white blood cell (WBC) counts were determined with a haemocytometer, packed cell volume (PCV) was by micro-haematocrit, haemoglobin (HGB) was determined using cyanomethaemoglobin method while mean cell volume (MCV) and mean cell haemoglobin (MCH) were calculated. The results obtained showed that, for Wistar rats fed with normal rat feed, the baseline values prior to the commencement of feeding trial for serum total protein, albumin, body weight, PCV, WBC, platelet count (PLT), RBC, HGB, lymphocyte count (LYM), MCH, MCV, neutrophil (NEU), and eosinophil (EOS) were 82.9 g/dL, 44.75 g/dL, 142.80g, 41.14%, 7.60×103 μL−1, 404.85×103 μL−1, 5.68×106 μL−1, 10.80 g/dL, 50.28 %, 18.42 pg, 59.28 fL, 45.57 %, and 2.57 % respectively. After the feeding trial, the serum total protein, albumin, body weight, PCV, WBC, PLT, RBC, HGB, LYM, MCH, MCV, NEU, and EOS in Wistar rats fed with the formulated infant food (the experimental group) were 79.6 g/dL, 50.65 g/dL, 169.18g, 40.14%, 5.77 ×103 μL−1, 309.85 ×103 μL−1, 6.85×106 μL−1, 12.45 g/dL, 52.85 %, 18.24 pg, 58.64 fL, 42.00 %, and 2.14% respectively and these were in most cases, either greater or comparable with their corresponding mean values of 69.1 g/dL, 44.40 g/dL, 177.97 g, 30.24%, 4.18×103 μL−1, 188.14×103 μL−1, 5.29×106 μL−1, 10.74 g/dL, 53.14 %, 20.37 pg, 57.61 fL, 38.85 %, and 4.57 % respectively in Wistar rats fed with the proprietary infant formula (the control group). This shows that the potential infant food has a more positive effect on most of the biological indices assessed than the proprietary infant food or a comparable effect at the least.\",\"PeriodicalId\":19641,\"journal\":{\"name\":\"Ovidius University Annals of Chemistry\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.0000,\"publicationDate\":\"2019-07-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Ovidius University Annals of Chemistry\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.2478/auoc-2019-0021\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"CHEMISTRY, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Ovidius University Annals of Chemistry","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2478/auoc-2019-0021","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
引用次数: 0
摘要
摘要研究了一种配方婴儿食品对Wistar大鼠某些生物学指标的性能评价。分别饲喂正常大鼠饲料、配方婴儿食品和专有食品的大鼠,用比色法测定血清总蛋白和白蛋白,用血细胞计测定红细胞(RBC)和差异白细胞(WBC)计数,用微红细胞压积法测定堆积细胞体积(PCV),用氰甲基血红蛋白法测定血红蛋白(HGB),同时计算平均细胞体积(MCV)和平均细胞血红蛋白(MCH)。结果表明,饲喂正常大鼠饲料的Wistar大鼠,试验开始前血清总蛋白、白蛋白、体重、PCV、WBC、血小板计数(PLT)、RBC、HGB、淋巴细胞计数(LYM)、MCH、MCV、中性粒细胞(NEU)和嗜酸性粒细胞(EOS)的基线值分别为82.9 g/dL、44.75 g/dL、142.80g、41.14%、7.60×103 μL−1、404.85×103 μL−1、5.68×106 μL−1、10.80 g/dL、50.28%、18.42 pg、59.28 fL、45.57%和2.57%。喂养试验后,血清总蛋白、白蛋白、体重,PCV,白细胞,血小板,红细胞,血红蛋白,LYM、妇幼保健、MCV、NEU,和EOS Wistar鼠美联储制定婴儿食品(实验组)79.6 g / dL, 50.65 g / dL, 169.18 g, 40.14%, 5.77×103μL−1,309.85×103μL−1,6.85×106μL−1,12.45 g / dL, 52.85%, 18.24 pg, 58.64 fL, 42.00%和2.14%,这是在大多数情况下,更大的或类似的相应的平均值为69.1 g / dL, 44.40 g / dL, 177.97克,分别为30.24%,4.18×103 μL−1,188.14×103 μL−1,5.29×106 μL−1,10.74 g/dL, 53.14%, 20.37 pg, 57.61 fL, 38.85%, 4.57%。这表明,潜在的婴儿食品对大多数生物指标的影响比专利婴儿食品更积极,或者至少有类似的影响。
Performance evaluation of a formulated infant food on some biological indices in Wistar rats
Abstract Performance evaluation of a formulated infant food on some biological indices in Wistar rats was investigated. For rats fed separately with normal rat feed, formulated infant food and proprietary food, serum total protein and albumin were determined by colorimetric method, red blood cell (RBC) and differential white blood cell (WBC) counts were determined with a haemocytometer, packed cell volume (PCV) was by micro-haematocrit, haemoglobin (HGB) was determined using cyanomethaemoglobin method while mean cell volume (MCV) and mean cell haemoglobin (MCH) were calculated. The results obtained showed that, for Wistar rats fed with normal rat feed, the baseline values prior to the commencement of feeding trial for serum total protein, albumin, body weight, PCV, WBC, platelet count (PLT), RBC, HGB, lymphocyte count (LYM), MCH, MCV, neutrophil (NEU), and eosinophil (EOS) were 82.9 g/dL, 44.75 g/dL, 142.80g, 41.14%, 7.60×103 μL−1, 404.85×103 μL−1, 5.68×106 μL−1, 10.80 g/dL, 50.28 %, 18.42 pg, 59.28 fL, 45.57 %, and 2.57 % respectively. After the feeding trial, the serum total protein, albumin, body weight, PCV, WBC, PLT, RBC, HGB, LYM, MCH, MCV, NEU, and EOS in Wistar rats fed with the formulated infant food (the experimental group) were 79.6 g/dL, 50.65 g/dL, 169.18g, 40.14%, 5.77 ×103 μL−1, 309.85 ×103 μL−1, 6.85×106 μL−1, 12.45 g/dL, 52.85 %, 18.24 pg, 58.64 fL, 42.00 %, and 2.14% respectively and these were in most cases, either greater or comparable with their corresponding mean values of 69.1 g/dL, 44.40 g/dL, 177.97 g, 30.24%, 4.18×103 μL−1, 188.14×103 μL−1, 5.29×106 μL−1, 10.74 g/dL, 53.14 %, 20.37 pg, 57.61 fL, 38.85 %, and 4.57 % respectively in Wistar rats fed with the proprietary infant formula (the control group). This shows that the potential infant food has a more positive effect on most of the biological indices assessed than the proprietary infant food or a comparable effect at the least.