以枣汁为碳源对豆科农杆菌本地分离株产凝乳的影响

Q4 Agricultural and Biological Sciences Basrah Journal of Agricultural Sciences Pub Date : 2023-06-28 DOI:10.37077/25200860.2023.36.1.13
Yaqob S. S. Al-Rumaidh, S. T. Al-Sahlany, Haider. I. Ali
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引用次数: 0

摘要

农杆菌负责生产被称为凝乳多糖的多糖。该菌株是从当地各种来源(包括农业土壤、根瘤和植物根系)收集的10株分离株中产生的。通过形态学、显微镜和生化试验鉴定分离株。在对分离株进行测试以确定哪一株能产生最多凝乳蛋白后,最终产生了A2分离株,其生产能力为29.2 g.L-1。实验结果表明,使用含有浓度为26.4 ml、100 mL-1的枣汁的改性培养基可提高凝乳素的产量。所得产量为30.7 g。L-1是可能的最高产量。利用红外光谱(FTIR)、核磁共振(NMR)和高效液相色谱(HPLC)等技术对其进行鉴定。该改性培养基具有利用农业废弃物生产凝乳的能力。
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Impact of Using Date Juice as a Carbon Source on Curdlan Produced by a Local Isolate of Agrobacterium leguminum
Agrobacterium species are responsible for the production of the polysaccharide known as curdlan. The curdlan was produced from 10 isolates that were collected from a variety of local sources, including as agricultural soils, root nodules, and plant roots. The isolates were identified by examinations using morphological, microscopic, and biochemical tests. After testing the isolates to see which ones could produce the most curdlan, the A2 isolate finally emerged with a production capacity of 29.2 g.L-1. According to the findings, the production of curdlan was increased using the modified medium that contained date juice at a concentration of 26.4 mL.100 mL-1 of the production medium. The resultant yield was 30.7 g.L-1, which was the highest possible yield. The identification of curdlan was validated by the utilization of FTIR, NMR, and HPLC techniques. The modified medium had the capability of being utilized in the production of curdlan from agricultural waste products.
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来源期刊
Basrah Journal of Agricultural Sciences
Basrah Journal of Agricultural Sciences Environmental Science-Pollution
CiteScore
1.20
自引率
0.00%
发文量
35
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