亚临界水萃取法和常规提取法提取的番荔枝叶提取物对其抗氧化活性、总多酚和芦丁含量进行了评价

IF 0.6 4区 综合性期刊 Q3 MULTIDISCIPLINARY SCIENCES Acta Scientiarum-technology Pub Date : 2022-08-26 DOI:10.4025/actascitechnol.v45i1.61153
R.T.S. Santos, Reinaldo Ricarte da Silva Junior, M. Ferreira, L. Soares, L. Stragevitch, Leandro Danielski
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引用次数: 0

摘要

番荔枝(番荔枝)是一种美洲本土植物,以其多叶的树冠和果实而闻名,在南美洲和东南亚广泛食用。采用索氏提取法、浸渍法、超声提取法和加压热水(亚临界)水提取法分别获得了番荔枝叶提取物,并对其抗氧化活性进行了评价。采用Folin-Ciocalteu法测定提取物的总多酚含量,DPPH法和ABTS法测定提取物的抗氧化活性,以评价和比较提取物的生物活性。采用高效液相色谱法对芦丁进行鉴定和定量,芦丁是一种特殊的类黄酮,是所有提取物中最具代表性的成分。芦丁以其潜在的天然抗氧化剂而闻名。在所有的提取方法中,以乙醇为溶剂的索氏法提取的总多酚含量较高,没食子酸当量/g含量为2.83%,并且具有较高的抗氧化活性。芦丁含量与抗氧化活性之间存在相关性。索氏提取显示,芦丁含量最高,为0.130±0.003 g g-1,这可能解释了所获得的木香叶提取物具有较高的抗氧化活性。
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Annona muricata leaf extracts obtained with subcritical water and conventional methods: evaluation of antioxidant activities, total polyphenol and rutin contents
Annona muricata (soursop) is a native American plant and is well known for its leafy canopy and its fruits, widely consumed in South America and Southeast Asia. The objective of this work was to obtain Annona muricata leaf extracts and to evaluate their antioxidant activities, which were obtained by four different extraction methods: Soxhlet, maceration, ultrasound, and pressurized hot (subcritical) water. In order to assess and compare the extracts bioactivities, total polyphenol contents of the extracts were evaluated by the Folin-Ciocalteu method and the antioxidant activities were determined by DPPH and ABTS methods. High performance liquid chromatography was used to identify and quantify rutin, a special flavonoid, which was found to be the most representative component, for all extracts obtained. Rutin is known for its benefits has a potential natural antioxidant. Among all extraction methods investigated, extracts obtained by the Soxhlet method using ethanol as solvent showed a higher total polyphenol content, 2.83% of gallic acid equivalent/g plant material, as well as higher antioxidant activity. A correlation between rutin content and antioxidant activity was found. Soxhlet extractions revealed samples with the highest rutin content of 0.130 ± 0.003 g g-1, what may explain the high antioxidant activity of the obtained A. muricata leaf extracts.
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来源期刊
Acta Scientiarum-technology
Acta Scientiarum-technology 综合性期刊-综合性期刊
CiteScore
1.40
自引率
12.50%
发文量
60
审稿时长
6-12 weeks
期刊介绍: The journal publishes original articles in all areas of Technology, including: Engineerings, Physics, Chemistry, Mathematics, Statistics, Geosciences and Computation Sciences. To establish the public inscription of knowledge and its preservation; To publish results of research comprising ideas and new scientific suggestions; To publicize worldwide information and knowledge produced by the scientific community; To speech the process of scientific communication in Technology.
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