磁性控制镁、钙、锌的核磁离子合成DNA作为一种强大而通用的杀伤癌细胞的手段

B. Anatoly, K. Dmitry
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引用次数: 1

摘要

DNA合成通常被认为是由Zn2+、Ca2+和Mg2+离子催化的亲核反应。这些具有非磁性核的离子被具有磁性核的离子取代,显示出巨大的同位素效应:磁性离子对DNA合成的抑制是非磁性离子的3-5倍。这一观察结果明确地证明了DNA的合成是通过自由基对机制进行的,这在化学中是众所周知的,并且表明自由基是通过反应伙伴之间的电子转移成对产生的。DNA合成的磁场依赖性令人信服地证明了自由基对机制,这种机制甚至在聚合酶链反应中也有体现。当至少两个离子进入催化位点时,这种机制,在能量尺度上比亲核机制便宜一个数量级。它与亲核机制几乎同等共存;它们的竞争是由离子浓度控制的。自由基对机制是由两种离子(磁性离子和非磁性离子)诱导的;唯一的区别是,它与磁性离子的效率提高了3-5倍。核磁离子25Mg2+、43Ca2+、67Zn2+使聚合酶的催化活性降低2-3倍,甚至降低30-50倍,增加癌细胞的死亡率。这些离子可以被认为是廉价、容易获得、安全(对免疫、信号和其他蛋白质系统没有影响)、强大和通用的抗癌手段,可以选择性地杀死任何类型的癌细胞。
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Magnetic Control of the DNA Synthesis by Nuclear Magnetic Ions of Mg, Ca and Zn as a Powerful and Universal Means to Kill Cancer Cells
DNA synthesis is commonly accepted to occur as a nucleophilic reaction catalyzed by Zn2+, Ca2+ and Mg2+ ions. The substitution of these ions with nonmagnetic nuclei by ions with magnetic nuclei was shown to produce a huge isotope effect: magnetic ions suppress DNA synthesis by 3–5 times with respect to nonmagnetic ones. This observation unambiguously evidences that the DNA synthesis occurs by radical pair mechanism, which is well known in chemistry and implies pair wise generation of radicals by electron transfer between reaction partners. Magnetic field dependence of the DNA synthesis convincingly proves radical pair mechanism, which manifests itself even in the polymerase chain reaction. This mechanism, being on the energy scale by order of magnitude cheaper that the nucleophilic one, is switched on, when at least two ions enter into the catalytic site. It coexists with nucleophilic mechanism almost on a par; their competition is controlled by concentration of ions. Radical pair mechanism is induced by both sorts of ions, magnetic and nonmagnetic; the only difference is that it functions by 3-5 times more efficiently with magnetic ions. Nuclear magnetic ions 25Mg2+, 43Ca2+, and 67Zn2+, decreasing catalytic activity of polymerase by 2-3 times, even more strongly, by 30-50 times, increase mortality of cancer cells. These ions may be considered as the cheap, easily available, safe (no influence on the immune, signaling and other protein systems), powerful, and universal anti-cancer means for selective killing cancer cells of any types.
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