Langmuir细菌-脂质单层的分子相互作用、弹性性质和纳米结构:解决细菌膜不对称之谜

IF 7.9 2区 化学 Q1 CHEMISTRY, PHYSICAL Current Opinion in Colloid & Interface Science Pub Date : 2023-10-01 DOI:10.1016/j.cocis.2023.101731
Xueying Guo, Wuge H. Briscoe
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引用次数: 0

摘要

革兰氏阴性菌(GNB)的膜特别坚固,这是由于额外的、独特的、高度不对称的外膜,以脂多糖(lps)为主要成分。这种脂多糖层作为抗抗生素、宿主免疫反应和其他环境应激的保护屏障。然而,为GNB细胞壁的基础研究构建包含LPS的模型膜以捕获结构不对称性仍然是一个开放的挑战。在此背景下,我们讨论了最近对Langmuir单层脂多糖的物理化学研究如何帮助我们更好地理解模型脂多糖细菌膜的弹性特性和结构完整性。经典的Langmuir-Blodgett槽被用来揭示含有不同分子结构的LPS突变体的单层的不同脂质相行为,以模拟GNB外膜的外小叶,揭示基础分子相互作用。抗菌肽的渗透和渗透可以改变LPS单层膜的粘弹性。含有lps的Langmuir单层也可以作为不对称固体支撑双层的外层叶转移到衬底上,我们将讨论这种方法的局限性和潜在的优化。最后,我们强调了不同的物理化学方法如何证实并有助于揭示模型细菌膜的结构特征。
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Molecular interactions, elastic properties, and nanostructure of Langmuir bacterial-lipid monolayers: Towards solving the mystery in bacterial membrane asymmetry

The membrane of Gram-negative bacteria (GNB) is especially robust due to the additional, unique, highly asymmetric outer membrane, with lipopolysaccharides (LPSs) as the main component. This LPS layer serves as a protective barrier against antibiotics, host immune responses, and other environmental stresses. However, constructing model membranes containing LPS that capture the structural asymmetry for fundamental studies of the GNB cell wall remains an open challenge. In this context, we discuss how recent physicochemical studies of Langmuir monolayers incorporating LPS help us better understand the elastic properties and structural integrity of model LPS bacterial membranes. The classic Langmuir–Blodgett trough has been used to reveal different lipid phase behaviors of monolayers containing LPS mutants with different molecular architectures to mimic the outer leaflet of the GNB outer membrane, shedding light on the underpinning molecular interactions. Permeation and penetration of antimicrobial peptides are shown to alter the viscoelastic properties of LPS monolayers. The LPS-containing Langmuir monolayer can also be transferred to a substrate as the outer leaflet of an asymmetric solid-supported bilayer, and we will discuss the limitations and potential optimization of this method. Finally, we highlight how different physicochemical methods can corroborate and contribute to unravelling the structural characteristics of model bacterial membranes.

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来源期刊
CiteScore
16.50
自引率
1.10%
发文量
74
审稿时长
11.3 weeks
期刊介绍: Current Opinion in Colloid and Interface Science (COCIS) is an international journal that focuses on the molecular and nanoscopic aspects of colloidal systems and interfaces in various scientific and technological fields. These include materials science, biologically-relevant systems, energy and environmental technologies, and industrial applications. Unlike primary journals, COCIS primarily serves as a guide for researchers, helping them navigate through the vast landscape of recently published literature. It critically analyzes the state of the art, identifies bottlenecks and unsolved issues, and proposes future developments. Moreover, COCIS emphasizes certain areas and papers that are considered particularly interesting and significant by the Editors and Section Editors. Its goal is to provide valuable insights and updates to the research community in these specialized areas.
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