来自泰国南邦Sob Prab合作有限公司的低等级菠萝和酸橙提取物的体外抗衰老活性

Korawinwich Boonpisuttinant, Somkit Unkeaw, Wirinda Chomphoo, Sarinporn Udompong, H. Y. Khong
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引用次数: 1

摘要

本研究以泰国南邦Sob Prab合作社有限公司的低档菠萝和青柠为研究对象,研究其抗衰老活性。榨出菠萝(PA‑J)和酸橙汁(LM‑J),然后用95% (v/v)乙醇(PA‑E和LM‑E)浸渍提取菠萝和酸橙渣,用蒸馏水(PA‑W和LM‑W)煮沸。所有提取物的提取率范围为14.58 ~ 23.01,其中萜类化合物作为植物化学物质存在。对于各提取物的抗衰老活性,发现LM-J提取物具有最高的抗氧化活性,包括自由基清除、金属螯合和脂质过氧化活性,而PA - E提取物对B16F10细胞具有酪氨酸酶抑制活性和抗黑素生成的美白作用,并对人真皮成纤维细胞具有胶原生物合成的刺激作用。此外,所有提取物在0.1 mg mL-1时对人真皮成纤维细胞均无细胞毒性。这项研究表明,PA‑E和LM提取物可能有利于成为抗衰老化妆品的活性成分。
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In vitro Anti-ageing Activities of the Extracts of Low-Grade Pineapple and Lime Key from Sob Prab Cooperative Limited, Lampang, Thailand
In this study, the low‑grade pineapple and lime key from Sob Prab Cooperative Limited, Lampang, Thailand were selected to investigate the anti‑ageing activities. The pineapple (PA‑J) and lime key juices (LM‑J) were squeezed and then the pineapple and lime key residues were extracted by maceration in 95% (v/v) ethanol (PA‑E and LM‑E) and boiled in distilled water (PA‑W and LM‑W). The extraction yields from all extracts ranged from 14.58 to 23.01, with the presence of terpenoids as a phytochemical. For anti-ageing activities of all extracts, it was found that the LM-J extract showed the highest antioxidant activity including free radical scavenging, metal chelation and lipid peroxidation activities, whereas the PA‑E extract exhibited the whitening effect including tyrosinase inhibition activity and anti-melanogenesis on B16F10 cells, and the stimulation of collagen biosynthesis on human dermal fibroblasts. In addition, all extracts did not show cytotoxicity on human dermal fibroblasts at 0.1 mg mL-1. This study suggested that the PA‑E and the LM extracts might be beneficial to be an active ingredient for anti-ageing cosmetics.
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