成年鸡肠内容物中乳酸菌菌株的新记录

Q4 Agricultural and Biological Sciences Basrah Journal of Agricultural Sciences Pub Date : 2022-10-15 DOI:10.37077/25200860.2022.35.2.14
A. Al-Salhi, S. Al-Shatty, Eman A. Al-Imara, Qutiba J. Al-Khfaji
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引用次数: 0

摘要

本研究于2020年11月15日至12月26日在巴士拉大学农学院食品科学系微生物学实验室进行,对健康成年鸡的消化部位空肠、回肠和盲肠的乳酸菌菌群进行了研究,并确定了不同类型家禽中乳酸菌含量最多的目标部位。结果,除了经过三次分离细菌菌落和用聚合酶链反应进行遗传鉴定外,还获得了7种新的乳酸菌,并在伊拉克和世界各地的国家生物技术信息中心(NCBI)登记为新的当地菌株,如下:干酪乳杆菌Al-Salhi-1、helveticus Lactobacillus Al-Salhi-2、plantitibacillus Al-Salhi-3、Limosilactobacillus reuteri菌株AhQuSa-1、Limosilactobacillus s.p .菌株AhQuSa-2、Limosilactobacillus salivarius菌株AhQuSa-3、Lactobacillus Johnsonii菌株AhQuSa-4。结果也显示出明显优势(P≤0.05)对数的乳酸菌数量为每个:空肠的鸟类繁殖在当地环境中,以及在群鸟类饲养在商业领域与回肠和盲肠相比,随着细菌群当地鸟类内容:6.52,5.21,4.15 (Cfu / g)为每个空肠、回肠和盲肠,分别在集团商业鸟到达:空肠、回肠、盲肠分别为6.35、5.02、3.92 (Cfu/g)。
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New Recording of Lactic Acid Bacteria Strains from the Contents of Adult Chicken Intestines
The study was conducted in the Microbiology Laboratory of the Department of Food Science at Basrah University's, Faculty of Agriculture from 15/11/2020 to 26/12/2020, to study the flora of lactic acid bacteria in digestive parts )Jejunum, Ileum, and Ceca (for healthy adult chickens and identifying the target part that contains the largest number of lactic acid bacteria for different types of poultry. As a result, as well as isolating bacterial colonies after three times, and being genetically Identified with PCR, seven new species of lactic acid bacteria were acquired and registered at the National Center for Biotechnology Information (NCBI) as new local strains, both in Iraq and around the world, as follows: Lactobacillus gasseri strain Al-Salhi-1, Lactobacillus helveticus strain Al-Salhi-2, Lactiplantibacillus plantarum strain Al-Salhi-3, Limosilactobacillus reuteri strain AhQuSa-1, Limosilactobacillus sp. strain AhQuSa-2, Ligilactobacillus salivarius strain AhQuSa-3, Lactobacillus Johnsonii strain AhQuSa-4. The results also showed a significant superiority (P≤0.05) in the logarithmic numbers of lactic acid bacteria in the jejunum for each of : the group of birds bred in local environments, as well as in the group of birds bred in commercial fields in comparison with the Ileum and Ceca, as the bacterial content was in the group of local birds : 6.52, 5.21, 4.15 (Cfu/g) for each of the jejunum, ileum and Ceca, respectively, while in the group of commercial birds it reached: 6.35, 5.02, 3.92 (Cfu/g) for each of the jejunum, ileum and Ceca, respectively.
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来源期刊
Basrah Journal of Agricultural Sciences
Basrah Journal of Agricultural Sciences Environmental Science-Pollution
CiteScore
1.20
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0.00%
发文量
35
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