Yuangang Liu, Yan Bai, Shi-Bin Wang, A. Chen, Wen-Guo Wu
{"title":"贮藏温度对brl -海藻酸盐- plo -海藻酸盐(APornA)微胶囊的影响","authors":"Yuangang Liu, Yan Bai, Shi-Bin Wang, A. Chen, Wen-Guo Wu","doi":"10.4172/1662-100X.1000110","DOIUrl":null,"url":null,"abstract":"To obtain long-term storage of cell-encapsulated alginate-PLO-alginate (APornA) microcapsules, the influences of storage temperatures on the microcapsule morphology, cell viability and metabolic activities were investigated. The results showed that the microcapsules exhibited the original morphological characteristics with smooth surface, good sphericity and overall integrity after 4 weeks preserved in liquid N2. 4°C and -20°C were presented to be the harsh conditions for cell propagation and the secretion of metabolic products compared to -80°C and -196°C. It can be concluded that lower temperature is beneficial for the cell recovery. These results supplied supplementary data for poly- L-ornithine (PLO) as a potential coating material in the future cell microencapsulated applications.","PeriodicalId":15198,"journal":{"name":"Journal of Biomimetics, Biomaterials and Tissue Engineering","volume":"1 1","pages":"1-4"},"PeriodicalIF":0.0000,"publicationDate":"2013-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Effect of the Storage Temperatures on BRL-encapsulated-alginate-PLO-alginate(APornA) Microcapsules\",\"authors\":\"Yuangang Liu, Yan Bai, Shi-Bin Wang, A. Chen, Wen-Guo Wu\",\"doi\":\"10.4172/1662-100X.1000110\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"To obtain long-term storage of cell-encapsulated alginate-PLO-alginate (APornA) microcapsules, the influences of storage temperatures on the microcapsule morphology, cell viability and metabolic activities were investigated. The results showed that the microcapsules exhibited the original morphological characteristics with smooth surface, good sphericity and overall integrity after 4 weeks preserved in liquid N2. 4°C and -20°C were presented to be the harsh conditions for cell propagation and the secretion of metabolic products compared to -80°C and -196°C. It can be concluded that lower temperature is beneficial for the cell recovery. These results supplied supplementary data for poly- L-ornithine (PLO) as a potential coating material in the future cell microencapsulated applications.\",\"PeriodicalId\":15198,\"journal\":{\"name\":\"Journal of Biomimetics, Biomaterials and Tissue Engineering\",\"volume\":\"1 1\",\"pages\":\"1-4\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2013-09-10\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Biomimetics, Biomaterials and Tissue Engineering\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.4172/1662-100X.1000110\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Biomimetics, Biomaterials and Tissue Engineering","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4172/1662-100X.1000110","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Effect of the Storage Temperatures on BRL-encapsulated-alginate-PLO-alginate(APornA) Microcapsules
To obtain long-term storage of cell-encapsulated alginate-PLO-alginate (APornA) microcapsules, the influences of storage temperatures on the microcapsule morphology, cell viability and metabolic activities were investigated. The results showed that the microcapsules exhibited the original morphological characteristics with smooth surface, good sphericity and overall integrity after 4 weeks preserved in liquid N2. 4°C and -20°C were presented to be the harsh conditions for cell propagation and the secretion of metabolic products compared to -80°C and -196°C. It can be concluded that lower temperature is beneficial for the cell recovery. These results supplied supplementary data for poly- L-ornithine (PLO) as a potential coating material in the future cell microencapsulated applications.
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