Optimization of in vitro conditions for embryo culture of Euryale ferox Salisb. (Makhana) using 2, 4-D as growth regulator

Culture conditions were optimized for high-frequency plant regulation via zygotic embryogenesis from cell culture of  Euryale ferox  (Makhana). Zygotic embryo developed into the yellowish globular structure and small callus followed by shoot formation at a frequency of 40%. When cultured on half-strength Murashige and Skoog (MS) medium, supplemented with 0, 0.1, 0.3, 0.5, 1.0, 1.5 mg/L of 2,4-D as growth regulator, the frequency of yellowish globular structure increased flabbily.