基于蜡质突变的玉米油中棕榈酸含量的遗传分析

D. Tymchuk
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摘要

的目标。糯玉米wx突变对棕榈酸盐含量影响的测定及该性状的遗传分析。方法。在为期两年的试验中,对10个无亲缘关系的普通型玉米自交系和10个携带wx突变的自交系进行了分析。用第二格里夫法对6个wx突变基因携带者的双列杂交进行了棕榈酸盐含量的遗传分析。采用Peysker气相色谱法测定棕榈酸盐含量。结果。wx突变玉米的油中棕榈酸甘油酯含量高于普通型玉米。无亲缘自交系wx突变携带者棕榈酸盐含量存在数量变异,范围为13.3% ~ 16.4%。糯玉米棕榈酸盐含量的遗传以不完全显性方式进行,加性效应对变异的贡献占主导地位。较高水平性状受多基因隐性等位基因控制。结论。结果表明,突变体wx基因在9号染色体上存在棕榈酸酯编码位点的空间连锁,其作用受到多基因复合物的修饰。
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Genetic analysis of palmytic acid content in the oil of maize based on Waxy mutation
Aim. Determination of the effect of wx mutation on the palmitate content and genetic analysis of this trait in waxy maize. Methods. In two-year experiments, 10 unrelated maize inbreds of the common type and 10 inbreds – carriers of the wx mutation were analyzed. Genetic analysis of palmitate content was carried out in the diallel crosses of 6 indreds – carriers of the wx mutation by the second Griffing method. The palmitate content was determined by Peysker’s gas chromatographic method. Results. Carriers of the wx mutation of maize exceeded the maize of common type in the content of glycerides of palmitic acid in oils. The content of palmitate in unrelated inbreds – carriers of the wx mutation was subjected to quantitative variability and ranged from 13.3 % to 16.4 %. Inheritance of palmitate content in the waxy corn was carrired out by the type of incomplete dominance with a predominant contribution of additive effects to the variation. A higher level of trait was controlled by recessive alleles of polygens. Conclusions. The obtained results suggest the presence in chromosome 9 of spatial linkage of the mutant wx gene with palmitate - coding locus, the effect of which is modified by a polygenic complex.
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