热探针测试:一种量化小鼠热爪退出阈值的新型行为分析

J. Deuis, I. Vetter
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引用次数: 48

摘要

啮齿类动物模型经常用于提高我们对疼痛分子机制的理解和开发新型镇痛药。因此,需要强有力的行为分析来量化对不同感觉模式(如热)的伤害性反应。在这里,我们描述了一种新的行为实验来量化小鼠的热爪退出阈值,称为热探针测试,并将其与其他常用的测量热阈值的方法进行了比较,即哈格里夫斯测试和动态和传统的热板测试。在热探针测试中,将一个直径为2.5 mm的微圆金属探针,在接触时以2.5℃/秒的速度加热,在~ 37℃的起始温度下,应用于小鼠后爪的足底表面,并自动记录发生退缩反应的温度,称为爪子退缩温度。热探针测试可有效量化角叉胶诱导炎症的热异常性疼痛(拔爪温度3 h:对侧,50.3±0.6°C;同侧,43.1±1.0℃),烧伤(拔爪温度3 d:对侧,50.8±0.5℃;同侧,43.2±0.6°C)和外用辣椒素后(拔爪温度:对照,49.7±0.6°C;辣椒素,44.8±1.2°C),得出与Hargreaves试验相当的结果。此外,热探针实验可以检测阿片介导的卡拉胶性炎症的镇痛作用。因此,热探针实验是一种新的行为学方法,可以有效地量化小鼠疼痛模型的热异常性疼痛和镇痛。
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The thermal probe test: A novel behavioral assay to quantify thermal paw withdrawal thresholds in mice
ABSTRACT Rodent models are frequently used to improve our understanding of the molecular mechanisms of pain and to develop novel analgesics. Robust behavioral assays that quantify nociceptive responses to different sensory modalities, such has heat, are therefore needed. Here, we describe a novel behavioral assay to quantify thermal paw withdrawal thresholds in mice, called the thermal probe test, and compared it with other methods commonly used to measure heat thresholds, namely the Hargreaves test and the dynamic and conventional hot plate tests. In the thermal probe test, a slightly rounded 2.5 mm diameter metal probe that heats on contact at a rate of 2.5°C/sec, is applied to the plantar surface of the hind paw in mice at a starting temperature of ∼37°C, and the temperature at which a withdrawal response occurs, designated as the paw withdrawal temperature, is automatically recorded. The thermal probe test is effective at quantifying thermal allodynia in carrageenan-induced inflammation (paw withdrawal temperature 3 h: contralateral, 50.3 ± 0.6°C; ipsilateral, 43.1 ± 1.0°C), burns injury (paw withdrawal temperature 3 d: contralateral, 50.8 ± 0.5°C; ipsilateral, 43.2 ± 0.6°C) and after topical capsaicin (paw withdrawal temperature: vehicle control, 49.7 ± 0.6°C; capsaicin, 44.8 ± 1.2°C), giving comparable results to the Hargreaves test. In addition, the thermal probe test can detect opioid mediated analgesia in carrageenan-induced inflammation. Therefore the thermal probe test is a novel behavioral assay effective for quantifying thermal allodynia and analgesia in mouse models of pain.
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