利用微流体设计改进自供电葡萄糖生物传感器系统

R. Escalona-Villalpando, A. Sandoval-García, S. Minteer, L. Arriaga, J. Ledesma-García
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摘要

一种自供电葡萄糖传感器(SPGS)系统集成了生物燃料电池(BFC)作为生物传感器运行的电源。SPGS使用葡萄糖作为定量分析物和燃料,因为它是一种与糖尿病有关的生物医学分子。在微流控装置中,葡萄糖氧化酶电极为阳极,漆酶电极为阴极,葡萄糖浓度在0 ~ 10 mM之间。在极化和电流曲线中,观察到在较高的葡萄糖浓度下增加,而在存在干扰的情况下不改变其操作。该结果表明,通过集成微流体装置,SPGS的性能得到了改善,增加了稳定性、检出限、定量、稳定性和灵敏度,可以通过BFC操作来定量葡萄糖。
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Improving a self-powered glucose biosensor system using a microfluidic design
A self-powered glucose sensor (SPGS) system integrates a biofuel cell (BFC) as a power source for operation of a biosensor. SPGS uses glucose as an analyte to be quantified and as a fuel, since it’s a molecule of biomedical interest because it’s related to diabetes. Glucose oxidase electrode-based was used in the anode and laccase based-electrode was used in the cathode within a microfluidic device where different glucose concentrations between 0 and 10 mM were used. In the polarization and current curves were observed an increase at a higher glucose concentration, without altering its operation in the presence interferences. This result indicated an improvement in the performance of an SPGS by integrating a microfluidic device, increasing the stability, limit of detection, quantification, stability and sensitivity for the quantification of glucose through the operation of a BFC.
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