D. Matatagui, J. Fontecha, M. J. Fernández, J. P. Santos, M. C. Horrillo, I. Gràcia, C. Cané
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Microfluidics applied to Love-wave devices to detect biological warfare agents in dynamic mode
A Love-wave device combined with a microfluidic chip of PDMS has been developed to detect biological warfare agents (BWA) in liquid media. It has been tested with the realization of two immunoassays: in the first one the bacteriophage M13 has been detected through the mouse monoclonal antibody, anti-M13 (AM13) and in the second one the detection has been carried out by means of the immunoreaction of the rabbit immunoglobulin (Rabbit IgG) with the goat anti-rabbit polyclonal antibody (GAR). The detections have been performed in a dynamic mode (continuous flow-through) in order to obtain a short response time. Different concentrations have been detected with a fast response and a good discrimination among them.
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