Thanh Hoa Mac Thi, Thanh Nguyen Ha, Ngoc Lan Dang Thi, Thanh Ha Pham Thi, Thanh An Vu Thi, Khanh Cao Cong
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High performance liquid chromatography analytical method for glutaraldehyde determination in disinfectants
Glutaraldehyde (GA) is commonly used to disinfect surfaces and equipment in industries, agriculture, healthcare, laboratories, etc. To evaluate the quality of products containing GA, a HPLC method using 2,4-diphenylhydrazine derivative was developed. The analyte was separated by CN column with isocratic of acetonitrile and phosphoric acid 0,1% (ratio 50 : 50) and detected by PDA detector using a wavelength of 360 nm. The method was validated for specificity, linear curves, precision and accuracy which meet the requirements of AOAC.
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