{"title":"应用生物发光方法研究应激对生命系统的影响","authors":"G. Lomakina","doi":"10.37747/2312-640x-2021-19-250-252","DOIUrl":null,"url":null,"abstract":"Bioluminescent methods based on firefly luciferase for detecting the effect of membrane-active agents (antibiotics and saponins) on living prokaryotic and eukaryotic cells have been developed. New data on the kinetics and mechanism of action of colistin on E. coli cells and digitonin cells on NEK293 cells were obtained. Examples of the use of constitutive and inducible luciferase reporters in cell biology are given.","PeriodicalId":13077,"journal":{"name":"http://eng.biomos.ru/conference/articles.htm","volume":"19 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"APPLICATION OF BIOLUMINESCENT METHODS TO STUDY STRESS EFFECTS ON LIVING SYSTEMS\",\"authors\":\"G. Lomakina\",\"doi\":\"10.37747/2312-640x-2021-19-250-252\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Bioluminescent methods based on firefly luciferase for detecting the effect of membrane-active agents (antibiotics and saponins) on living prokaryotic and eukaryotic cells have been developed. New data on the kinetics and mechanism of action of colistin on E. coli cells and digitonin cells on NEK293 cells were obtained. Examples of the use of constitutive and inducible luciferase reporters in cell biology are given.\",\"PeriodicalId\":13077,\"journal\":{\"name\":\"http://eng.biomos.ru/conference/articles.htm\",\"volume\":\"19 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2021-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"http://eng.biomos.ru/conference/articles.htm\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.37747/2312-640x-2021-19-250-252\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"http://eng.biomos.ru/conference/articles.htm","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.37747/2312-640x-2021-19-250-252","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
APPLICATION OF BIOLUMINESCENT METHODS TO STUDY STRESS EFFECTS ON LIVING SYSTEMS
Bioluminescent methods based on firefly luciferase for detecting the effect of membrane-active agents (antibiotics and saponins) on living prokaryotic and eukaryotic cells have been developed. New data on the kinetics and mechanism of action of colistin on E. coli cells and digitonin cells on NEK293 cells were obtained. Examples of the use of constitutive and inducible luciferase reporters in cell biology are given.
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