{"title":"以克鲁维酵母为原料生产重组酵母凝乳酶的研究","authors":"S. Belenkaya, V. V. Elchaninov","doi":"10.37747/2312-640x-2021-19-331-332","DOIUrl":null,"url":null,"abstract":"A plasmid vector pSVB was developed, which allows integrating an expression cassette containing the maral prochymosin gene into the genome of the yeast Kluyveromyces lactis. Producer strains have been obtained that ensure the accumulation of recombinant maral chymosin in the culture medium with a milk-clotting activity of up to 145 AU / ml.","PeriodicalId":13077,"journal":{"name":"http://eng.biomos.ru/conference/articles.htm","volume":"5 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"DEVELOPMENT OF A PRODUCER OF RECOMBINANT MARAL CHYMOSIN BASED ON THE YEAST KLUYVEROMYCES LACTIS\",\"authors\":\"S. Belenkaya, V. V. Elchaninov\",\"doi\":\"10.37747/2312-640x-2021-19-331-332\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"A plasmid vector pSVB was developed, which allows integrating an expression cassette containing the maral prochymosin gene into the genome of the yeast Kluyveromyces lactis. Producer strains have been obtained that ensure the accumulation of recombinant maral chymosin in the culture medium with a milk-clotting activity of up to 145 AU / ml.\",\"PeriodicalId\":13077,\"journal\":{\"name\":\"http://eng.biomos.ru/conference/articles.htm\",\"volume\":\"5 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2021-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"http://eng.biomos.ru/conference/articles.htm\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.37747/2312-640x-2021-19-331-332\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"http://eng.biomos.ru/conference/articles.htm","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.37747/2312-640x-2021-19-331-332","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 1
摘要
建立了一种质粒载体pSVB，将含有酵母原凝乳酶基因的表达盒整合到酵母的基因组中。已经获得了生产菌株，确保在培养基中积累重组马尔凝乳酶，凝乳活性高达145 AU / ml。
DEVELOPMENT OF A PRODUCER OF RECOMBINANT MARAL CHYMOSIN BASED ON THE YEAST KLUYVEROMYCES LACTIS
A plasmid vector pSVB was developed, which allows integrating an expression cassette containing the maral prochymosin gene into the genome of the yeast Kluyveromyces lactis. Producer strains have been obtained that ensure the accumulation of recombinant maral chymosin in the culture medium with a milk-clotting activity of up to 145 AU / ml.
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