连续制备用于代谢分析的真核细胞的微流控装置

2013 IEEE 26th International Conference on Micro Electro Mechanical Systems (MEMS 2013) : Taipei, Taiwan, 20-24 January 2013. IEEE International Conference on Micro Electro Mechanical Systems (26th : 2013 : Taipei, Taiwan) Pub Date : 2013-03-07 DOI:10.1109/MEMSYS.2013.6474227

N. Rajabi, J. Bahnemann, T. Tzeng, A. Zeng, J. Muller

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引用次数: 3

摘要

一种新型芯片实验室提出了高通量制备哺乳动物细胞样品。微流控芯片由两个温度区和五个功能模块组成，共有四个流体入口通道和三个样品采集出口通道。芯片的关键模块是用于生物系统扰动的快速分裂-重组微混合器，用于降低停留时间分布的混沌平流“搅拌”孵化通道，以及用于高效颗粒分离和介质交换的螺旋通道。该无源芯片适用于细胞室内代谢分析的动态脉冲实验。

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Microfluidic device for the continuous preparation of eukaryotic cells for metabolic analysis

A novel lab-on-a-chip is presented for the high-throughput preparation of mammalian cell samples. The microfluidic chip consists of two temperature zones and five functional modules with a total number of four fluidic inlet and three outlet channels for sample collection. The key modules of the chip are a rapid split-and-recombine micromixer for biological system perturbation, a chaotic advection “stirred” incubation channel for lowered residence time distribution, and a spiral channel for efficient particle separation and media exchange. The passive chip is suitable for dynamic pulse experiments for metabolic analyses in cell compartments.

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2013 IEEE 26th International Conference on Micro Electro Mechanical Systems (MEMS 2013) : Taipei, Taiwan, 20-24 January 2013. IEEE International Conference on Micro Electro Mechanical Systems (26th : 2013 : Taipei, Taiwan)

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