{"title":"油菜籽中中性酚类化合物的分离、分离及高效液相色谱分析","authors":"B. Matthäus","doi":"10.1002/(SICI)1521-3803(199804)42:02<75::AID-FOOD75>3.3.CO;2-6","DOIUrl":null,"url":null,"abstract":"HPLC has been applied for the analysis of neutral phenolic compounds in rapeseed. Extraction of phenolic compounds with 70% (v/v) methanol, separation of neutral and acidic phenolic compounds by solid phase extraction with a C 18 -cartridge and HPLC separation of the extracts so obtained are described. Additionally, a rapeseed extract was fractionated by a simple chromatographic method using Sephadex LH-20. Six main compounds from the extract were isolated. A further purification was performed by preparative HPLC and a C 18 -cartridge are used to isolate the neutral phenolic compounds. An attempt to identify the compounds was made from the UV-spectra corresponding to each isolated peak of the chromatogram.","PeriodicalId":18955,"journal":{"name":"Nahrung-food","volume":"4 1","pages":"75-80"},"PeriodicalIF":0.0000,"publicationDate":"1998-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"13","resultStr":"{\"title\":\"Isolation, fractionation and HPLC analysis of neutral phenolic compounds in rapeseeds\",\"authors\":\"B. Matthäus\",\"doi\":\"10.1002/(SICI)1521-3803(199804)42:02<75::AID-FOOD75>3.3.CO;2-6\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"HPLC has been applied for the analysis of neutral phenolic compounds in rapeseed. Extraction of phenolic compounds with 70% (v/v) methanol, separation of neutral and acidic phenolic compounds by solid phase extraction with a C 18 -cartridge and HPLC separation of the extracts so obtained are described. Additionally, a rapeseed extract was fractionated by a simple chromatographic method using Sephadex LH-20. Six main compounds from the extract were isolated. A further purification was performed by preparative HPLC and a C 18 -cartridge are used to isolate the neutral phenolic compounds. An attempt to identify the compounds was made from the UV-spectra corresponding to each isolated peak of the chromatogram.\",\"PeriodicalId\":18955,\"journal\":{\"name\":\"Nahrung-food\",\"volume\":\"4 1\",\"pages\":\"75-80\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1998-04-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"13\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Nahrung-food\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1002/(SICI)1521-3803(199804)42:02<75::AID-FOOD75>3.3.CO;2-6\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nahrung-food","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1002/(SICI)1521-3803(199804)42:02<75::AID-FOOD75>3.3.CO;2-6","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Isolation, fractionation and HPLC analysis of neutral phenolic compounds in rapeseeds
HPLC has been applied for the analysis of neutral phenolic compounds in rapeseed. Extraction of phenolic compounds with 70% (v/v) methanol, separation of neutral and acidic phenolic compounds by solid phase extraction with a C 18 -cartridge and HPLC separation of the extracts so obtained are described. Additionally, a rapeseed extract was fractionated by a simple chromatographic method using Sephadex LH-20. Six main compounds from the extract were isolated. A further purification was performed by preparative HPLC and a C 18 -cartridge are used to isolate the neutral phenolic compounds. An attempt to identify the compounds was made from the UV-spectra corresponding to each isolated peak of the chromatogram.
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