离体心肌细胞舒张粘弹性的定量测定

C. Baicu, M. Zile
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引用次数: 0

摘要

导致舒张性充血性心力衰竭(CHF)的病理过程,如压力过载肥厚(POH),会导致心肌细胞(心肌细胞)物质特性的异常,并可能选择性地改变其弹性刚度、粘度或两者兼有。以前用于表征这些心肌细胞粘弹性特性的方法受到特定生物学和工程限制的限制,这使得无法在模拟正常生理的条件下进行测试。目前的研究提出了一种单轴可变速率拉伸方法,其中将分离的心肌细胞嵌入三维凝胶基质中进行拉伸。生理Ca++ (2.5 mM)和高达100 μm/sec的快速拉伸速率提供了与体内生理平行的实验条件。所提出的方法识别和单独量化细胞刚度和粘度,并表明POH增加了弹性和粘性心肌细胞舒张特性。
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Quantification of Diastolic Viscoelastic Properties of Isolated Cardiac Muscle Cells
Pathological processes which cause diastolic congestive heart failure (CHF), such as pressure overload hypertrophy (POH), produce abnormalities in the material properties of cardiac muscle cells (cardiomyocytes) and may selectively alter its elastic stiffness, viscosity, or both. Previous methods used to characterize these cardiomyocyte viscoelastic properties were constrained by specific biological and engineering limitations, which prevented testing in conditions that mimic normal physiology. The current study proposes an uniaxial variable-rate stretching method, in which isolated cardiomyocytes embedded in a three-dimensional gel matrix were subjected to stretch. Physiological Ca++ (2.5 mM) and rapid stretch rates up to 100 μm/sec provided experimental conditions parallel to in vivo physiology. The proposed method identified and individually quantified both cellular stiffness and viscosity, and showed that POH increased both elastic and viscous cardiomyocyte diastolic properties.
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