植物原料颜色对含花青素提取物稳定性的光度测定指标

IF 1 Q4 CHEMISTRY, MULTIDISCIPLINARY Ovidius University Annals of Chemistry Pub Date : 2020-07-01 DOI:10.2478/auoc-2020-0016
V. Koldaev, Artem Yurevich Manyakhin, P. S. Zorikov
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引用次数: 0

摘要

摘要本文旨在分光光度法测定可提取花青素在植物物料干燥过程中随颜色变化的稳定性变化。研究使用了25科50种植物的生的和干的有色部分。提取液在95%乙醇溶液中用盐酸酸化(pH ~ 1)制备,吸收光谱在210 ~ 680 nm范围内。用消光变异性系数、强度吸收相对系数和广义稳定系数来测定花色苷的降解。酒红色和紫罗兰色果壳提取物的稳定系数最高,为0.934±0.024 ~ 0.973±0.024,而相同颜色花瓣提取物的稳定系数为1.19 ~ 1.44倍。黑色、红色和蓝色材料的稳定系数分别是紫红色和酒红色材料的1.15 ~ 1.19倍、1.74 ~ 2.48倍和4.65 ~ 4.84倍。将本研究中采用的花色苷稳定性的分光光度因子应用于工业栽培植物中花色苷类中药制剂原料的选择是合适的。最稳定的花青素是紫红色和黑色水果中的花青素。
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Spectrophotometric indicators of the stability of anthocyanin-containing extracts depending on the color of plant materials
Abstract This paper aims at spectrophotometric determination of changes in stability of extractable anthocyanins during drying of plant materials depending on their color. Raw and dried colored parts of 50 plant species from 25 families were used for the study. The extracts were prepared over 95% ethanol acidified with hydrochloric acid (pH ~ 1). The absorption spectra were registered within the range of 210 to 680 nm. The extinction variability factor, coefficient of intensity absorption relative and generalized stability factor were used to determine the anthocyanin degradation. The highest values of the stability factor were obtained for the extracts from fruit shells of burgundy or violet color within the range of 0.934±0.024 to 0.973±0.024, while the extracts from flower petals of the same care featured the stability factor that was 1.19 to 1.44 times less. The values of the stability factor of the extracts from black, red and blue materials are 1.15 to 1.19 times, 1.74 to 2.48 times and 4.65 to 4.84 times less respectively than those of the extracts from violet-burgundy materials. It is appropriate to apply the spectrophotometric factors of anthocyanins stability used in this study to selection of promising plants for industrial cultivation as material of anthocyanin-containing herbal preparations. The most stable anthocyanins are those of burgundy-purple and black fruits.
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来源期刊
Ovidius University Annals of Chemistry
Ovidius University Annals of Chemistry CHEMISTRY, MULTIDISCIPLINARY-
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11.10%
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20
审稿时长
5 weeks
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