Enzymatic Screening of β-Amyloid Precursor Protein-Based Substrates

We performed an enzymatic screening of synthetic peptides based on β-amyloid precursor protein substrates. The template peptide sequence was a decapeptide derived from our previous screening study, which determined several effective unnatural amino acids. In this study, new libraries containing some unnatural amino acid compounds were prepared in the solid phase and digested with the β-site amyloid precursor protein-cleaving enzyme. The reaction mixture was analyzed using high-performance liquid chromatography combined with mass spectrometry. The peptides that showed a higher cleavage than the template sequence were determined and reported.