控制番茄植物细胞中brsno类固醇和乙烯功能的基因表达依赖于叶片的引力刺激。

S. V. Sukhaveyeva, A. M. Каbachevskay, N. A. Radtsevich, I. D. Volotovski
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引用次数: 0

摘要

研究了重力刺激对番茄叶片细胞在向地性反应早期(15分钟- 3小时)和后期(超过3小时- 24小时)基因表达水平的影响。利用实时RT-PCR技术测定油菜素内酯和乙烯功能相关基因编码蛋白表达的敏感性。用乙烯利(外源乙烯的来源)预处理番茄植株后,这些基因在重力刺激下的相对表达量增加。
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ЗАВИСИМОСТЬ ЭКСПРЕССИИ ГЕНОВ, КОНТРОЛИРУЮЩИХ ФУНКЦИОНИРОВАНИЕ БРАССИНОСТЕРОИДОВ И ЭТИЛЕНА В КЛЕТКАХ РАСТЕНИЙ ТОМАТА, ОТ ГРАВИСТИМУЛЯЦИИ ИХ ВЕРХУШЕЧНЫХ ЛИСТЬЕВ
The influence of gravistimulation on the gene expression level in tomato leaf cells at early (15 minutes – 3 hours) and late (more than 3 hours – 24 hours) stages of the gravitropic response was assessed. The sensitivity of expression of genes coding proteins associated with brassinosteroid and ethylene functioning was determined using real-time RT-PCR. The pre-treatment of tomato plants with ethephon (the source of exogenous ethylene) led to an increase in the relative level of expression of the investigated genes under gravistimulation in the plants.
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