不同培养基对褐藻生长和碳水化合物含量的影响

C. S. Arsule, B. Sonawane
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摘要

采集方法:采用手术刀采集5 ~ 10 cm土层土壤样品。土壤样本收集在6 × 4英寸大小的聚乙烯袋中。摘要:从不同地点采集的土壤样品中分离到念珠菌。采用Desikachary(1959)的形态学变异和分类学方法进行鉴定。在实验室中获得了诺斯托克菌的无菌培养。生物量生产采用BG-11培养基、Fogg培养基、Allen and Arnon培养基、Zarrouk培养基和CFTRI培养基。采用双层平纹棉布过滤收获生物质,风机干燥。收获后,获得的生物量进行生长分析。碳水化合物总量采用Anthrone法估算(Hedge and Hofreiter, 1962)。在使用的不同培养基中,BG-11培养基比其他培养基更能支持Nostoc的生长。总碳水化合物含量在CFTRI培养基中最高,Allen和Arnon培养基次之。©2019 igglobal研究与出版基金会。所有
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Influence of Various Culture Media on Growth and Carbohydrates Content in a Cyanobacterium Nostoc muscorum
Method of collection: The soil samples from 5-10 cm deep soil layers were collected using the scalpels. Soil samples were collected in polythene bags of size 6 x 4 inches. ABSTRACT: Nostoc muscorum was isolated from the collected soil samples from different locations. Identification was carried out using morphological variation and taxonomical approaches according to Desikachary (1959). The axenic culture of Nostoc muscorum was obtained in the laboratory. For the biomass production, different culture media were used namely BG-11, Fogg’s medium, Allen and Arnon medium, Zarrouk’s medium and CFTRI medium. The biomass was harvested by filtration through double layered muslin cloth and dried using air blower. After harvesting, the biomass obtained was subjected to the growth analysis. The total carbohydrates were estimated by following Anthrone method (Hedge and Hofreiter, 1962). Out of the different culture media used, BG-11 medium supported the growth of Nostoc muscorum properly as compared to other media used. The total carbohydrates content was more in Nostoc muscorum grown in CFTRI medium followed by the Allen and Arnon medium. © 2019 iGlobal Research and Publishing Foundation. All
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