用卡尔斯伯根酵母菌发酵啤酒废谷所得的生物乙醇保存水果

Q3 Agricultural and Biological Sciences Revista Facultad Nacional de Agronomia Medellin Pub Date : 2020-09-01 DOI:10.15446/rfnam.v73n3.85316
C. O. Ogidi, Oluwatobiloba Hannah George, O. Aladejana, Olu Malomo, O. Famurewa
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引用次数: 1

摘要

啤酒精(BSG)是啤酒酿造过程中产生的可再生木质纤维素生物质。它是各种生物技术应用的基础。以BSG为主要底物,利用卡尔斯伯根酵母进行深层发酵生产生物乙醇。为生产生物乙醇进行了糖化和发酵研究。在29±2℃、180转/分的搅拌速度下,将50 g L-1 BSG装入灭菌发酵罐。将pH调至6.0后,加入500 mL酵母培养物,在优化条件下浸泡7天。发酵产物在66±1℃的旋转蒸发器浓缩,用重铬酸盐法对乙醇进行定性测定。生物乙醇收率为22%,比重为0.8,温度为28°C。傅里叶变换红外光谱(FTIR)证实了生物乙醇中存在-CH3、-OHstretch和-CH2stretch。对于防腐剂的检测,葡萄球菌、欧文氏菌、乳酸杆菌、芽孢杆菌、黄单胞菌、假单胞菌、微球菌和棒状杆菌是从奥孙州不同地区的水果中分离到的细菌。分离到的真菌属有曲霉属、炭疽菌属、青霉属、镰刀菌属、Alternaria菌属、根霉属、念珠菌属、酵母菌属、地毛菌属和毕赤酵母属。BSG生产的生物乙醇对微生物生长的抑制范围为7.0 ~ 11.5 mm,因此可以保存选定的果实。因此,可以采用微生物发酵农工废弃物技术,将废弃生物质转化为有用资源。
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Fruit preservation with bioethanol obtained from the fermentation of brewer’s spent grain with Saccharomyces carlsbergensis
Brewer’s Spent Grain (BSG) is renewable lignocellulosic biomass generated from the beer brewing process. It serves as a substrate for various biotechnological applications. BSG was used as the main substrate for bioethanol production with Saccharomyces carlsbergensis in submerged fermentation. Saccharification and fermentation studies were performed for the production of bioethanol. A sterilized fermenter was loaded with 50 g L-1 of BSG at 29±2 °C and an agitation speed of 180 rpm. pH was adjusted to 6.0 before the addition of 500 mL of yeast culture for 7 days under submerged and optimized conditions. The fermented product was concentrated using a rotary evaporator at 66±1 °C, and ethanol was qualitatively determined by the dichromate method. Bioethanol yield was 22%, with a specific gravity of 0.8 at 28 °C. Fourier-Transform Infrared Spectroscopy (FTIR) confirmed the presence of -CH3 stretch, -OHstretch and -CH2stretch in bioethanol. For the preservative test, Staphylococcus spp., Erwinia spp., Lactobacillus spp., Bacillus spp., Xanthomonas spp., Pseudomonas spp., Micrococcus spp. and Corynebacterium spp. were the bacteria isolated from fruits examined from different regions of Osun State. The genera of fungi isolated were Aspergillus, Colletotrichum, Penicillium, Fusarium, Alternaria, Rhizopus, Candida, Saccharomyces, Geotrichium and Pichia. Bioethanol produced from BSG inhibited the growth of microorganisms with zones of inhibition range from 7.0 mm to 11.5 mm, and thus, selected fruits were preserved. Hence, the fermentation technology of agro-industrial wastes with microorganisms can be adopted to convert waste biomass to useful resources.
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来源期刊
Revista Facultad Nacional de Agronomia Medellin
Revista Facultad Nacional de Agronomia Medellin Agricultural and Biological Sciences-Agricultural and Biological Sciences (miscellaneous)
CiteScore
1.30
自引率
0.00%
发文量
34
审稿时长
16 weeks
期刊介绍: La Revista Facultad Nacional de Agronomía Medellín (RFNA), de la Universidad Nacional de Colombia, Sede Medellín, Facultad de Ciencias Agrarias, es una publicación científica editada desde el año 1939, en formato impreso y digital y de acceso abierto desde la web —sin costo para autores y lectores—. Está orientada a investigadores que crean conocimiento disciplinar y multidisciplinar que articula la ciencia y la tecnología para hacer más productivo el campo a nivel empresarial y de economía campesina. La Revista tiene como objetivo divulgar resultados de investigación mediante artículos originales, inéditos y arbitrados (peer review) de carácter científico que respondan a preguntas específicas y que proporcionen sustento y pruebas a una hipótesis, en aspectos relacionados con las Ciencias Agronómicas, Producción animal, Ciencias Forestales, Ingeniería Agrícola, de Alimentos y otras afines que contribuyan a la solución de los limitantes del agro a nivel nacional, regional e internacional. La Revista recibe y publica, sin ningún costo, artículos en idioma inglés de investigación, revisión, reseñas y cartas al editor. Desde el 2017 la periodicidad de publicación es cuatrimestral, se publican tres números al año con circulación nacional e internacional, en los siguientes períodos: Enero - Abril Mayo - Agosto Septiembre - Diciembre This journal does not charge APCs or submission charges.
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