A highly performed enzymatic biosensor using distributed electrodes decorated with hydrothermally treated reduced graphene oxide and platinum nanoparticles

In this work, hydrothermally reduced graphene oxide (TRGO) was developed with environment friendly reducing agent by optimum time due to exfoliate easily in solution. Two distributed sensing electrodes connected in series are decorated with TRGO added platinum nanoparticles (PtNP) and enzyme composites with nafion, which integrated in a single chip for effectively glucose sensing. The fabricated biosensor exhibited good amperometric response to glucose with a sensitivity of 16.22 μA/mMcm2, low detection limit of 0.001 mM (signal to noise ratio is 3), and a short response time of 3 s. Moreover, the effect of interference materials and the stability of the biosensor were also investigated.