常规血液学检查在室温下的血液样本的稳定性使用了球根露

Gilang Nugraha, Nuroktya Ningsih, Titik Sulifah, Sitti Fitria
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引用次数: 1

摘要

全血细胞计数(CBC)是最常受医生影响的实验室检查之一。使用血液学分析仪提供了更广泛的探针参数范围。预分析阶段占误差的70%,其中之一是检查的延迟。报告结果的变化是由于EDTA添加剂和室温引起的血细胞形态的变化。本研究的目的是在室温下使用血液分析仪检测血液样品中各种CBC参数的稳定性的干扰。这项实验实验室研究是在Pramita Jemur Andayani临床实验室进行的。从志愿者身上采集血液样本,保存在室温下,立即进行检查(对照组),并在延迟6、12和24小时后进行检查(治疗组)。共有30名受访者,包括8名男性和22名女性。受访者平均年龄22±1岁。两组患者红细胞、Hct、MCV、MCHC、PLT、PDW检查结果差异无统计学意义(p值0.05)。Hgb、MCH、RDW、WBC、NEU、IG、MONO、EO、BASO、LYM、PLT、PDW检查结果均< 0.05。使用CELL-DYN Ruby血液学分析仪进行延迟CBC检查,直接在6小时延迟检查的几个参数上给出不同的结果。
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STABILITAS PEMERIKSAAN HEMATOLOGI RUTIN PADA SAMPEL DARAH YANG DIDIAMKAN PADA SUHU RUANG MENGGUNAKAN CELL-DYN RUBY
Complete blood count (CBC) is one of the laboratory tests most often influenced by doctors. The use of a hematology analyzer offers a wider range of probe parameters. The pre-analytic stage accounts for 70% of errors, one of which is the delay of the examination. Changes in report results were reported due to changes in blood cell morphology due to EDTA additives and room temperature. The aim of this research is the disturbance of stability of the results of examination of various CBC parameters in blood samples that are left at room temperature for 24 hours using a hematology analyzer. This experimental laboratory research was conducted at the Pramita Jemur Andayani Clinical Laboratory. Blood samples were obtained from volunteers, stored at room temperature and subjected to immediate examination (control) and after a delay of 6, 12 and 24 hours (treatment). A total of 30 respondents, consisting of 8 men and 22 women. The mean age of the respondents was 22 ± 1 year. There was no difference in the results of the examination (p-value > 0.05) in the RBC, Hct MCV, MCHC, PLT and PDW. The results of the examination (p-value < 0.05) were found on Hgb, MCH, RDW, WBC, NEU, IG, MONO, EO, BASO, LYM, PLT and PDW. Delayed CBC examinations using the CELL-DYN Ruby hematology analyzer directly gave different results on several parameters ranging from 6 hours delay of examination.
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