{"title":"合成量子点在细胞成像中的应用","authors":"Hengyi Xu, Feng Xu, Yonghua Xiong, C. Wan, Jingfei Zhang, Hua Wei, Jiang Zhu","doi":"10.1109/SOPO.2009.5230150","DOIUrl":null,"url":null,"abstract":"Quantum dots (QDs) have received considerable attention due to their advantages and are widely used in biological studies, especially for multiplexed staining assays and immunological assays. Here we report an easy method for quantum dot synthesis and encapsulation, and use for efficient bioconjugation with secondary antibody. For the application of QDs-antibody conjugates, we approached a fast dot blotting immunological assay, and then we used a breast cancer cell line SKBR3 and anti-EpCAM antibody as the primary antibody for the cell imaging. The results shows that this conjugates can be successfully used in immunological assays such as dot blotting and cell imaging, and with the help of certain blocking buffers, specific and sensitive signals can be obtained.","PeriodicalId":6416,"journal":{"name":"2009 Symposium on Photonics and Optoelectronics","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2009-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Application of Synthesized Quantum Dots for Cell Imaging\",\"authors\":\"Hengyi Xu, Feng Xu, Yonghua Xiong, C. Wan, Jingfei Zhang, Hua Wei, Jiang Zhu\",\"doi\":\"10.1109/SOPO.2009.5230150\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Quantum dots (QDs) have received considerable attention due to their advantages and are widely used in biological studies, especially for multiplexed staining assays and immunological assays. Here we report an easy method for quantum dot synthesis and encapsulation, and use for efficient bioconjugation with secondary antibody. For the application of QDs-antibody conjugates, we approached a fast dot blotting immunological assay, and then we used a breast cancer cell line SKBR3 and anti-EpCAM antibody as the primary antibody for the cell imaging. The results shows that this conjugates can be successfully used in immunological assays such as dot blotting and cell imaging, and with the help of certain blocking buffers, specific and sensitive signals can be obtained.\",\"PeriodicalId\":6416,\"journal\":{\"name\":\"2009 Symposium on Photonics and Optoelectronics\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2009-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"2009 Symposium on Photonics and Optoelectronics\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1109/SOPO.2009.5230150\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"2009 Symposium on Photonics and Optoelectronics","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1109/SOPO.2009.5230150","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
摘要
量子点(Quantum point, QDs)由于其自身的优点,在生物学研究中得到了广泛的应用,特别是在多重染色和免疫学研究中。本文报道了一种简单的量子点合成和封装方法,并将其用于与二抗的高效生物偶联。对于qds抗体偶联物的应用,我们采用快速点印迹免疫检测方法,然后使用乳腺癌细胞系SKBR3和抗epcam抗体作为细胞成像的一抗。结果表明,该偶联物可成功应用于点阵印迹和细胞成像等免疫分析,并在一定的阻断缓冲液的帮助下,可获得特异性和敏感性的信号。
Application of Synthesized Quantum Dots for Cell Imaging
Quantum dots (QDs) have received considerable attention due to their advantages and are widely used in biological studies, especially for multiplexed staining assays and immunological assays. Here we report an easy method for quantum dot synthesis and encapsulation, and use for efficient bioconjugation with secondary antibody. For the application of QDs-antibody conjugates, we approached a fast dot blotting immunological assay, and then we used a breast cancer cell line SKBR3 and anti-EpCAM antibody as the primary antibody for the cell imaging. The results shows that this conjugates can be successfully used in immunological assays such as dot blotting and cell imaging, and with the help of certain blocking buffers, specific and sensitive signals can be obtained.