植物育种特点及抗旱机理

R. Kovbasenko, A. Dmitriev, S. A. Polyakovsky
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摘要

的目标。提出了利用选择因子对番茄植株进行抗旱细胞选择的方案。方法。以番茄品种斯维塔诺克为研究对象。根据已知方法进行体外细胞培养、无菌种子萌发、微克隆繁殖、愈伤组织发生和形态发生。结果。过氧化物酶在刺激保护机制中的作用已经建立,植物激素在这些过程中的作用已经揭示。结果表明,在番茄细胞离体培养的逐步筛选中,筛选出耐盐的TTP-1和TTP-2。研究了信号分子处理过程中过氧化物酶对植物抗性生长的影响。结论。研究表明,信号分子积极参与植物在大气和土壤干旱胁迫下的抗氧化保护。我们使用的信号分子在应激条件下具有保护作用:特殊特征是在各种因素的不利影响后恢复过程中非常活跃的一部分。抗氧化酶过氧化物酶活性的增加也有助于激活番茄植株对高温的系统抗性。
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The peculiarities of plant breeding and drought-resistance mechanisms
Aim. To propose a plan of cellular selection of tomato plants for resistance to drought with the use of selective factor is manit. Methods. The object of study was the tomato variety Svitanok. Work with cell culture in vitro, aseptic seed germination, microclonal reproduction, callusogenesis and initiation of morphogenesis were performed according to known methods. Results. The influence of peroxidase in the stimulation of protective mechanisms has been established and the role of phytohormones in these processes has been revealed. It was found that when using stepwise selection of tomato cell culture in vitro, samples of TTP-1 and TTP-2, tolerant to salinity, were selected and worked on. The effect of peroxidase on the growth of plant resistance during foliar treatment with signaling molecules is also shown. Conclusions. It has been shown that signaling molecules are actively involved in the antioxidant protection of plants under stress caused by air and soil drought. The signaling molecules we use have a protective effect under stress: specialty characteristic is a very active part in the recovery process after the adverse effects of various factors. The increase in the activity of the antioxidant enzyme peroxidase also contributes to the activation of the systemic resistance of tomato plants against high temperatures.
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