紫荆水提物多酚类物质的纳米包封及生物可及性

IF 4.1 Q2 FOOD SCIENCE & TECHNOLOGY Food Chemistry Molecular Sciences Pub Date : 2022-12-30 DOI:10.1016/j.fochms.2022.100144
Bárbara Verônica Cardoso de Souza , Mariana de Morais Sousa , José Augusto Gasparotto Sattler , Ana Cristina Sousa Gramoza Vilarinho Santana , Rusbene Bruno Fonseca de Carvalho , José de Sousa Lima Neto , Fernando de Matos Borges , Iramaia Angelica Neri Numa , Alessandra Braga Ribeiro , Lívio César Cunha Nunes
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引用次数: 4

摘要

紫荆是一种富含多酚的植物,主要用于其降糖活性,这与它的抗氧化和抗炎潜力有关。然而,这些生物活性化合物的有益作用直接取决于它们的生物可及性和生物利用度,这就需要能够提高和保持其生物活性的加工技术。采用喷雾干燥的方法,制备了福尔菲达叶浸膏(ESIN)和煎剂(ESDC)的纳米胶囊提取物。所使用的包封剂为麦芽糖糊精和胶体二氧化硅。采用HPLC-PDA-ESI-IT-MSn对制备的纳米胶囊进行表征,并对其模拟消化后的生物可及性和抗氧化活性进行了评价。此外,对纳米胶囊提取物进行了广泛的物理化学表征,并对其稳定性和工艺参数进行了评估。ESIN和ESDC提取物的产率分别为57.3%和62.7%,平均纳米胶囊尺寸为0.202 μm和0.179 μm,湿度和水活度较低(<0.5),粉末密度和流动性能良好(Hausner比≤1.25;卡尔指数18 - 19%)。扫描电镜显示其球形和非晶态形貌和低粘度,这可能有利于其溶解度。纳米胶囊的酚类化合物在400℃后降解,表现出较高的热稳定性。红外光谱鉴定出麦芽糖糊精和酚类化合物的存在,两者之间没有发生反应。色谱法证实了酚类化合物的存在,主要是黄酮醇及其o糖基化衍生物,以及碳水化合物,可能是麦芽糊精。体外消化模拟实验表明,ESIN和ESDC纳米胶囊的多酚和黄酮类化合物在胃相(多酚含量分别为49.38%和64.17%,黄酮类化合物含量分别为64.08%和36.61%)和十二指肠相(多酚含量分别为52.68%和79.06%,黄酮类化合物含量分别为13.24%和139.03%)具有生物可及性,ORAC法测定的抗氧化活性在胃消化后维持在52.27%到70.55%之间,在十二指肠消化后维持在25%之间。因此,福福卡塔提取物的纳米胶囊化是保存其生物活性化合物的可行选择,使其具有生物可及性和抗氧化活性,这使得它们适合掺入各种营养保健品配方,如胶囊、片剂和小袋。
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Nanoencapsulation and bioaccessibility of polyphenols of aqueous extracts from Bauhinia forficata link

Bauhinia forficata Link is a plant rich in polyphenols that has been used mainly for its hypoglycemic activity, which is related to its antioxidant and anti-inflammatory potential. However, the beneficial effect of these bioactive compounds is directly dependent on their bioaccessibility and bioavailability, requiring processing techniques that can improve and preserve their biological activities. This work aimed to obtain nanocapsulated extracts from the infusion (ESIN) and decoction (ESDC) of B. forficata Link leaves, by spray drying. The encapsulating agents used were maltodextrin and colloidal silicon dioxide. The nanocapsules were characterized by HPLC-PDA-ESI-IT-MSn, evaluated the bioaccessibility of polyphenols after simulated digestion and their antioxidant activity. Additionally, an extensive physicochemical characterization of the nanocapsulated extracts was carried out and their stability and technological parameters were evaluated. The ESIN and ESDC extracts had yields of 57.3 % and 62.7 %, with average nanocapsules sizes of 0.202 μm and 0.179 μm, low humidity and water activity (<0.5), powder density and proper flow properties (Hausner ratio ≤ 1.25; Carr index 18–19 %). Scanning electron microscopy showed a spherical and amorphous morphology and low viscosity, which may have favored the solubility profile. The phenolic compounds of the nanocapsules degraded after 400 °C, showing high thermal stability. The infrared spectra identified the presence of maltodextrin and phenolic compounds and that there were no reactions between them. Chromatography confirmed the presence of phenolic compounds, mainly flavonols and their O-glycosylated derivatives, as well as carbohydrates, probably maltodextrin. Simulated in vitro digestion showed that polyphenols and flavonoids from ESIN and ESDC nanocapsules were bioaccessible after the gastric phase (49.38 % and 64.17 % of polyphenols and 64.08 % and 36.61 % of flavonoids) and duodenal (52.68 % and 79.06 % of polyphenols and 13.24 % and 139.03 % of flavoids), with a variation from 52.27 % to 70.55 % of the antioxidant activity maintained, by the ORAC method, after gastric digestion and still 25 %, after duodenal. Therefore, the nanoencapsulation of extracts of B. forficata is a viable option for the preservation of their bioactive compounds, making them bioaccessible and with antioxidant activity, which make them suitable for incorporation into various nutraceutical formulations, such as capsules, tablets and sachets.

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来源期刊
Food Chemistry Molecular Sciences
Food Chemistry Molecular Sciences Agricultural and Biological Sciences-Food Science
CiteScore
6.00
自引率
0.00%
发文量
83
审稿时长
82 days
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