格鲁吉亚的火灾

Dali L. Gaganidze , Mariam A. Aznarashvili , Tinatin A. Sadunishvili , Ekaterine O. Abashidze , Manana A. Gureilidze , Eter S. Gvritishvili
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引用次数: 11

摘要

火疫病是果树病害中危害最大的一种。大约180种蔷薇科的栽培和野生植物受到火疫病的危害。木瓜、苹果和梨最易患此病。目前,该病发生在欧洲和亚洲的40多个国家。火疫病造成的经济损失不仅表现在作物损失上,而且对整个果树园造成灭顶之灾的威胁。淀粉Erwinia amylovora是果树火疫病的致病菌,被列入A2检疫生物名录。2016年,格鲁吉亚农业部实验室植物害虫诊断部门的员工在姆茨赫塔地区的苹果幼苗中检测到淀粉状欧文菌。格鲁吉亚农业部国家食品局向粮农组织通报了病原体检测情况。本研究的目的是在格鲁吉亚东部(Kvemo Kartli、Shida Kartli和Kakheti)和西部(Imereti)地区收集的有火疫病嫌疑的果树样本中,用分子PCR方法检测淀粉状Erwinia amylovora细菌。利用特异引物,采用实时荧光定量PCR和常规PCR方法检测到淀粉状Erwinia amylovora菌,并在Shida Kartli的23份植物材料(11份苹果、6份梨和6份柑橘)、Kvemo Kartli的5份植物材料(1份柑橘和4份苹果)、Kakheti地区的2份苹果和Imereti (Zestafoni)地区的1份梨中鉴定出该菌。
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Fire blight in Georgia

Fire blight is distinguished among the fruit tree diseases by harmfulness. Fire blight damages about 180 cultural and wild plants belonging to the Rosaceae family. Quince, apple and pear are the most susceptible to the disease. At present, the disease occurs in over 40 countries of Europe and Asia. Economic damage caused by fire blight is expressed not only in crop losses, but also, it poses threat of eradication to entire fruit tree gardens. Erwinia amylovora, causative bacteria of fire blight in fruit trees, is included in the A2 list of quarantine organisms. In 2016, the employees of the Plant Pest Diagnostic Department of the Laboratory of the Georgian Ministry of Agriculture have detected Erwinia amylovora in apple seedlings from Mtskheta district. National Food Agency, Ministry of Agriculture of Georgia informed FAO on pathogen detection. The aim of the study is detection of the bacterium Erwinia amylovora by molecular method (PCR) in the samples of fruit trees, suspicious on fire blight collected in the regions of Eastern (Kvemo Kartli, Shida Kartli and Kakheti) and Western Georgia (Imereti).

The bacterium Erwinia amylovora was detected by real time and conventional PCR methods using specific primers and thus the fire blight disease confirmed in 23 samples of plant material from Shida Kartli (11 apples, 6 pear and 6 quince samples), in 5 samples from Kvemo Kartli (1 quince and 4 apple samples), in 2 samples of apples from Kakheti region and 1 sample of pear collected in Imereti (Zestafoni).

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