CRISPR-Cas9基因组编辑揭示了甲氧丁烯在黄热病蚊子,埃及伊蚊中的作用模式。

IF 3.7 4区 生物学 Q2 GENETICS & HEREDITY CRISPR Journal Pub Date : 2022-12-01 DOI:10.1089/crispr.2022.0066
Guan-Heng Zhu, Sharath Chandra Gaddelapati, Yaoyu Jiao, Jinmo Koo, Subba Reddy Palli
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引用次数: 0

摘要

甲基戊二烯是一种类似幼体激素(JH)的物质,广泛用于昆虫防治,但其作用方式尚不清楚。为了研究甲氧丁烯在黄热病蚊子埃及伊蚊中的作用,使用CRISPR-Cas9系统敲除E93(表皮激素诱导转录因子)。E93突变体蛹保留的幼虫组织与处理过甲氧苯醚的昆虫相似。这些昆虫完成蛹蜕化并作为蛹死亡。此外,转录因子broad复合物和kr ppel同源物1 (Kr-h1)的表达增加,程序性细胞死亡(PCD)和自噬基因的表达减少。这些数据表明,甲氧二烯通过JH受体、甲氧二烯耐受性起作用,诱导Kr-h1的表达,从而抑制E93的表达,导致PCD阻滞和幼虫组织自噬。幼虫组织的清除和成虫结构的形成失败会导致它们死亡。这些结果回答了长期以来关于甲氧丁烯作用方式的问题。
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CRISPR-Cas9 Genome Editing Uncovers the Mode of Action of Methoprene in the Yellow Fever Mosquito, Aedes aegypti.

Methoprene, a juvenile hormone (JH) analog, is widely used for insect control, but its mode of action is not known. To study methoprene action in the yellow fever mosquito, Aedes aegypti, the E93 (ecdysone-induced transcription factor) was knocked out using the CRISPR-Cas9 system. The E93 mutant pupae retained larval tissues similar to methoprene-treated insects. These insects completed pupal ecdysis and died as pupa. In addition, the expression of transcription factors, broad complex and Krüppel homolog 1 (Kr-h1), increased and that of programmed cell death (PCD) and autophagy genes decreased in E93 mutants. These data suggest that methoprene functions through JH receptor, methoprene-tolerant, and induces the expression of Kr-h1, which suppresses the expression of E93, resulting in a block in PCD and autophagy of larval tissues. Failure in the elimination of larval tissues and the formation of adult structures results in their death. These results answered long-standing questions on the mode of action of methoprene.

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来源期刊
CRISPR Journal
CRISPR Journal Biochemistry, Genetics and Molecular Biology-Biotechnology
CiteScore
6.30
自引率
2.70%
发文量
76
期刊介绍: In recognition of this extraordinary scientific and technological era, Mary Ann Liebert, Inc., publishers recently announced the creation of The CRISPR Journal -- an international, multidisciplinary peer-reviewed journal publishing outstanding research on the myriad applications and underlying technology of CRISPR. Debuting in 2018, The CRISPR Journal will be published online and in print with flexible open access options, providing a high-profile venue for groundbreaking research, as well as lively and provocative commentary, analysis, and debate. The CRISPR Journal adds an exciting and dynamic component to the Mary Ann Liebert, Inc. portfolio, which includes GEN (Genetic Engineering & Biotechnology News) and more than 80 leading peer-reviewed journals.
期刊最新文献
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