细菌提取物生物合成纳米银的优化及其抑菌潜力

Q1 Immunology and Microbiology Biotechnology Reports Pub Date : 2023-10-29 DOI:10.1016/j.btre.2023.e00816

Iván Solís-Sandí , Sara Cordero-Fuentes , Reinaldo Pereira-Reyes , José Roberto Vega-Baudrit , Diego Batista-Menezes , Gabriela Montes de Oca-Vásquez

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引用次数: 0

摘要

本研究以铜芽孢杆菌(Cupriavidus necator)、巨芽孢杆菌(Bacillus megaterium)和枯草芽孢杆菌(Bacillus subtilis)的上清液和胞内提取物为原料合成纳米银颗粒(AgNPs)。利用紫外可见光谱(UV-Vis)、红外光谱(FTIR)、DLS和TEM对AgNPs进行了表征。采用reazurin微滴板法测定AgNPs对大肠杆菌的抑菌作用。紫外可见光谱峰位于414 ~ 460 nm之间。TEM分析表明，合成的AgNPs主要呈球形。DLS测定的尺寸范围为20.8 ~ 118.4 nm。用上清液合成的AgNPs抗菌活性最高，而用细胞内提取物合成的AgNPs抗菌活性最高。因此，确定细菌种类，温度，pH值和提取物类型(上清液或细胞内)影响生物合成。因此，该合成方法为生产可用作抗菌剂的AgNPs提供了一种简单、环保、低成本的方法。

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Optimization of the biosynthesis of silver nanoparticles using bacterial extracts and their antimicrobial potential

In the present study, silver nanoparticles (AgNPs) were biosynthesized using the supernatant and the intracellular extract of Cupriavidus necator, Bacillus megaterium, and Bacillus subtilis. The characterization of the AgNPs was carried out using UV–Vis spectroscopy, FTIR, DLS and TEM. Resazurin microtiter-plate assay was used to determine the antimicrobial action of AgNPs against Escherichia coli. UV–Visible spectra showed peaks between 414 and 460 nm. TEM analysis revealed that the synthesized AgNPs showed mostly spherical shapes. DLS results determined sizes from 20.8 to 118.4 nm. The highest antimicrobial activity was obtained with the AgNPs synthesized with supernatant rather than those using the intracellular extract. Therefore, it was determined that the bacterial species, temperature, pH, and type of extract (supernatant or intracellular) influence the biosynthesis. This synthesis thus offers a simple, environmentally friendly, and low-cost method for the production of AgNPs, which can be used as antibacterial agents.

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来源期刊

Biotechnology Reports Immunology and Microbiology-Applied Microbiology and Biotechnology

CiteScore

15.80

自引率

0.00%

发文量

审稿时长

55 days

期刊介绍： Biotechnology Reports covers all aspects of Biotechnology particularly those reports that are useful and informative and that will be of value to other researchers in related fields. Biotechnology Reports loves ground breaking science, but will also accept good science that can be of use to the biotechnology community. The journal maintains a high quality peer review where submissions are considered on the basis of scientific validity and technical quality. Acceptable paper types are research articles (short or full communications), methods, mini-reviews, and commentaries in the following areas: Healthcare and pharmaceutical biotechnology Agricultural and food biotechnology Environmental biotechnology Molecular biology, cell and tissue engineering and synthetic biology Industrial biotechnology, biofuels and bioenergy Nanobiotechnology Bioinformatics & systems biology New processes and products in biotechnology, bioprocess engineering.