鼻内给药脂肪源性干细胞能触及并影响老年Wistar大鼠远端器官的组织结构吗?

Ghada Galal Hamam, Nevine Bahaa, Mona H Raafat
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摘要

干细胞治疗是治疗各种退行性疾病的一种非常有前途的策略。鼻内给药干细胞可以被认为是一种非侵入性的治疗选择。然而,关于干细胞到达远处器官的能力存在很大的争论。在这种情况下,尚不清楚它们是否能减轻这些器官中与年龄相关的结构变化。目的:本研究的目的是评估经鼻给药的脂肪源性干细胞(ADSCs)在不同时间间隔到达大鼠远端器官的能力,并探讨其对这些器官年龄相关结构变化的影响。材料与方法:本研究选用雌性Wistar大鼠49只,其中成年大鼠7只(6月龄),老年大鼠42只(2岁)。将大鼠分为三组:ⅰ组(成年对照组)、ⅱ组(老龄组)和ⅲ组(老龄ADSCs处理组)。ⅰ、ⅱ组大鼠于实验开始后15 d处死。III组大鼠经鼻灌胃ADSCs,分别于2 h、1 d、3 d、5 d、15 d后处死。收集心脏、肝脏、肾脏和脾脏标本,进行H和E、CD105免疫组织化学和免疫荧光技术处理。进行形态计量学研究和统计学分析。结果:经鼻给药2小时后,所有器官均出现ADSCs。在给药第3天达到最高值,到第15天免疫荧光逐渐减少,几乎消失。鼻内给药后第5天,一些与年龄相关的肾脏和肝脏结构恶化得到改善。结论:经鼻给药后,ADSCs可有效到达心脏、肝脏、肾脏和脾脏。ADSCs改善了这些器官中一些与年龄相关的变化。
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Can Intranasal Administration of Adipose-Derived Stem Cells Reach and Affect the Histological Structure of Distant Organs of Aged Wistar Rat?

Introduction: Stem cell therapy is a highly promising strategy in various degenerative diseases. Intranasal administration of stem cells could be considered as a non-invasive treatment option. However, there is great debate concerning the ability of stem cells to reach distant organs. It is also unclear in such a case if they can alleviate age-related structural changes in these organs.

Aim: The aim of this study is to evaluate the ability of intranasal administration of adipose-derived stem cells (ADSCs) to reach distant organs of rats at different time intervals and to investigate their effects on age-related structural changes in these organs.

Materials and methods: Forty-nine female Wistar rats were used in this study, seven of which were adults (6-month-old) and 42 were aged (2-year-old). Rats were divided into three-groups: Group-I (adult control), Group-II (aged), and Group-III (aged ADSCs treated). Rats of Groups I and II were sacrificed after 15 days from the beginning of the experiment. Rats of Group III were treated with intranasal ADSCs and were sacrificed after 2-h, 1-day, 3-day, 5-day, and 15-day. Heart, liver, kidney, and spleen specimens were collected and processed for H and E, CD105 immunohistochemistry, and immunofluorescent techniques. Morphometric study and statistical analysis were performed.

Results: ADSCs appeared in all organs examined after 2-h of intranasal administration. Their maximum presence was detected after 3-day of administration, after which their immunofluorescence gradually decreased and nearly disappeared from these organs by the 15th day. Improvement of some age-related deterioration in the structure of the kidney and liver occurred at day 5 after intranasal administration.

Conclusions: ADSCs effectively reached the heart, liver, kidney, and spleen after intranasal administration. ADSCs ameliorated some age-related changes in these organs.

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