苦艾提取物对喹啉酸诱导的OLN-93细胞损伤有减弱作用。

Q3 Pharmacology, Toxicology and Pharmaceutics Current drug discovery technologies Pub Date : 2023-01-01 DOI:10.2174/1570163820666230330105331

Roghayeh Rashidi, Maryam Akaberi, Aida Gholoobi, Hamed Ghazavi, Fatemeh Forouzanfar

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引用次数: 1

摘要

目的:在许多神经退行性疾病中发现喹啉酸(QA)积累增加。艾草(a.a absinthium)具有神经保护和抗氧化活性。本研究旨在探讨苦艾草对qn -93细胞神经毒性的影响。方法:将OLN-93细胞培养于含有10% (v/v)胎牛血清、100单位/ml青霉素、100 μg/ml链霉素的DMEM培养基中。用苦艾草提取物预处理细胞2 h，再用QA处理24 h。24 h后，测定细胞活力，测定细胞中丙二醛(MDA)、活性氧(ROS)和凋亡细胞的水平。结果:苦艾草提取物对OLN-93细胞有明显的抑制作用。苦艾草提取物预处理可减少qa损伤的OLN-93细胞的ROS生成、脂质过氧化和细胞凋亡。结论:苦艾草提取物通过氧化应激和细胞凋亡调节对qa诱导的神经毒性具有神经保护作用。

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Artemisia Absinthium Extract Attenuates the Quinolinic Acid-Induced Cell Injury in OLN-93 Cells.

Objective: Increased quinolinic acid (QA) accumulation has been found in many neurodegenerative diseases. Artemisia absinthium (A. absinthium) has been reported to have neuroprotective and antioxidant activities. This study was designed to evaluate the effect of A. absinthium in QAinduced neurotoxicity in OLN-93 Cells.

Methods: OLN-93 cells were cultured in a DMEM medium containing 10% (v/v) fetal bovine serum, 100 units/ml penicillin, and 100 μg/ml streptomycin. The cells were pretreated with concentrations of A. absinthium extract for two h and then exposed to QA for 24 h. After 24 h cell viability, the level of malondialdehyde (MDA), reactive oxygen species (ROS), and apoptotic cells were quantitated in OLN-93 Cells.

Results: Pretreatment with A. absinthium extract prevented the loss of cell viability in OLN-93 cells. ROS generation, lipid peroxidation, and apoptosis in QA-injured OLN-93 cells were reduced following A. absinthium extract pretreatment.

Conclusion: A. absinthium extract exerts its neuroprotective effect against QA-induced neurotoxicity via oxidative stress and apoptosis modulation.

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来源期刊

Current drug discovery technologies Pharmacology, Toxicology and Pharmaceutics-Drug Discovery

CiteScore

3.70

自引率

0.00%

发文量

期刊介绍： Due to the plethora of new approaches being used in modern drug discovery by the pharmaceutical industry, Current Drug Discovery Technologies has been established to provide comprehensive overviews of all the major modern techniques and technologies used in drug design and discovery. The journal is the forum for publishing both original research papers and reviews describing novel approaches and cutting edge technologies used in all stages of drug discovery. The journal addresses the multidimensional challenges of drug discovery science including integration issues of the drug discovery process.