黑木耳中黑色素的分离、表征及抗氧化活性研究。

IF 1.4 4区 生物学 Q4 MYCOLOGY International journal of medicinal mushrooms Pub Date : 2023-01-01 DOI:10.1615/IntJMedMushrooms.2023048271
Qianwen Shi, Zeen Yang, Renhui Fan, Jialei Chu, Chenlu Fang, Yusi Zhang, Wenting Shi, Yongjun Zhang
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引用次数: 0

摘要

黑木耳子实体的细胞壁非常坚韧,用传统的制备方法很难溶解黑色素。为了考察黑色素的高效制备及其抗氧化应激能力,本文首先利用超声辅助碱性纤维素酶,在单因素实验的基础上,通过Box-Behnken设计优化最佳破壁参数。优化后,木耳中黑色素的得率为3.201±0.018%。然后,采用不同类型、不同比例的深共晶溶剂(DES)进行进一步萃取。选择氯化胆碱与尿素的比例为1:2时,黑色素得率可达25.99%±2.36%。扫描电镜(SEM)显示黑色素为无定形物质,无晶体结构。x射线光电子能谱(XPS)分析显示,黑色素主要由C(5.38%)、O(15.69%)和N(30.29%)组成,为真黑色素的典型成分。黑色素与ABTS+和羟自由基清除能力均呈浓度依赖性;在0.5 mg/mL浓度下,与阴性对照相比,显著延长了秀丽隐杆线虫在过氧化氢和紫甲基胁迫下的存活时间,提高了体内谷胱甘肽水平和酶(总超氧化物歧化酶和过氧化氢酶)活性(P < 0.05),表明黑色素增强了秀丽隐杆线虫抗氧化应激能力。
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Isolation, Characterization, and Antioxidant Activity of Melanin from Auricularia auricula (Agaricomycetes).

The cell wall of Auricularia auricula fruit bodies is extremely tough, making it difficult to dissolve the melanin using the traditional preparation method. To investigate the efficient preparation of melanin and its resistance to oxidative stress, this paper first used ultrasound-assisted alkaline cellulase to optimize the optimal wall-breaking parameters through a Box-Behnken design based on a single-factor experiment. After optimization, the yield of melanin from A. auricula reached 3.201 ± 0.018%. Then, different types and different proportions of deep eutectic solvents (DES) were used for further extraction. When choline chloride and urea were selected and the ratio was 1:2, the melanin yield was up to 25.99% ± 2.36%. Scanning electron microscope (SEM) images showed that the melanin was amorphous mass with no crystal structure. X-ray photoelectron spectroscopy (XPS) analysis revealed that the melanin was mainly composed of C (5.38%), O (15.69%) and N (30.29%), as was the typical composition of eumelanin. The melanin had a concentration-dependent relationship with both ABTS+ and hydroxyl radical scavenging ability; at the concentration of 0.5 mg/mL, it significantly prolonged Caenorhabditis elegans survival under hydrogen peroxide and methyl viologen stress and increased the glutathione level and enzyme (total superoxide dismutase and catalase) activities in vivo compared with the negative control (P < 0.05), indicating that the melanin enhances oxidative stress resistance in C. elegans.

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来源期刊
CiteScore
2.60
自引率
16.70%
发文量
91
审稿时长
6-12 weeks
期刊介绍: The rapid growth of interest in medicinal mushrooms research is matched by the large number of disparate groups that currently publish in a wide range of publications. The International Journal of Medicinal Mushrooms is the one source of information that will draw together all aspects of this exciting and expanding field - a source that will keep you up to date with the latest issues and practice. The International Journal of Medicinal Mushrooms published original research articles and critical reviews on a broad range of subjects pertaining to medicinal mushrooms, including systematics, nomenclature, taxonomy, morphology, medicinal value, biotechnology, and much more.
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