Identification and rapid screen based on immune sensor

Abstract

The object of this paper is that the immobilization technology for antibodies onto silicon-based chip. The active site of an IgG, the paratope, in many cases does not interact with the solid matrix and is, therefore, available for antibody-antigen complex formation. Due to △S > 0, the spontaneity activation of the Sulfur-Au bond is successfully forming and the target protein binding region was chemically modified to introduce aldehyde group. We also demonstrated the more linkers, the more antibodies onto the surface. And than we use fluorescence substrate, Rodamine, conjugate the antibodies in order to observe the amount of antiboies. In our studies, we also demanded that our methodology is still good to antibodies immobilization without any impair the antibodies. Although there is still non-specific binding of Ab on the modify chip, we can observe the antibodies (Green flourescence) on the measure of areas which is the metal, Au deposited on modify chip.