Detection of pathogenic bacteria using immunofiltration and lightscribe technology

Abstract

The detection of pathogenic bacteria is key for the identification and prevention and of problems related to health and safety. Accurate and quick detection and quantification of pathogen microorganisms in food, drinking water and in clinical diagnostics using cost-effective methodologies are greatest challenges currently facing food industry, health and environmental fields. A rapid and sensitive biosensor, based on immunological methods, was developed for bacteria detection using LightScribe disc as platform. We are able to detect between 0 and 106 CFU/mL in only 1.5 hours without the need for sample pre-concentration. The cross-reactivity study and individual ready-to-eat meat samples were inoculated with live bacteria to demonstrate proof-of-concept for real samples.