The Effectiveness of Ethanolic Extract of Centella asiatica (L.) on Healing Minor Recurrent Aphthous Stomatitis in Wistar Male Rats (Rattus norvegicus)

Abstract

One of the most common oral diseases is Recurrent Aphthous Stomatitis (RAS) and the most common form is Minor RAS. Gotu kola leaves ( Centella asiatica (L.) Urban) contain active components that can help the healing process that widely used as traditional medicine. The aim of this study was to determine the effectiveness of the 70% ethanolic extract of C. asiatica on the healing of Minor RAS. This study is a true experimental with a Pretest-Posttest Randomized Control Group design. The purposive sampling technique is used. The sample of this study are 24 male wistar rats divided into 4 sample groups, a negative control group and 3 experimental groups (treatment with ethanolic extract of C. asiatica at a concentration of 25%, 50%, and 75%). The formation of the lesion minor RAS was by making a lesion on the lower labial oral mucosa of the rats by using a 10% H 2 O 2 solution, then giving the treatment in each group for 7 days twice a day. Observations were made by measuring the diameter of minor RAS lesions in all samples using a sliding caliper every morning for 7 days. This research conducted in August 2021. The results of the measurement of lesion diameter were tested by the Shapiro-Wilk normality test. One Way ANOVA test was performed, the results showed that there was no significant difference in the diameter of the lesion between experimental groups and negative control group (p>0.05). Ethanolic extract of C. asiatica did not significantly heal minor RAS lesions.