Perfil diferencial de transcritos em endométrio eutópico de mulheres inférteis com endometriose e controles durante a janela de implantação

Abstract

Objectives

Molecular alterations in the eutopic endometrium of women with endometriosis may be involved in the endometriosis‐related infertility. This study aimed to compare the differentially expressed genes (DEG) in eutopic endometrium of infertile women with endometriosis, infertile controls (IC; male and/or tubal factor) and fertile controls (FC) through RNA‐Seq.

Material and methods

Endometrial biopsies were obtained from 17 patiens (6 infertile women with endometriosis, 6 IC and 5 FC) during the implantation window. The RNA was extracted and the RNA‐Seq was performed at a HISEQ 2500 Illumina Platform, high output, paired end. Standardization and differential expression were conducted in the statistical R environment using DESeq2 package.

Results

The groups IC and FC were similar. No DEG has been identified comparing CF and endometriosis groups. Five DEGs (SCUBE1, CCL20, LGALS9 C, TRIM 29 e WNT11) were identified in the advanced endometriosis (EIII/IV) group, and 1 (KANSL1‐AS1) in the initial endometriosis (EI/II) group compared to FC. Two DEGs (KANSL1‐AS1 and VGLL3) were identified by comparing EI/II and EIII/IV groups.

Conclusions

These data suggest that the eutopic endometrium of infertile women with endometriosis, especially those with advanced disease, may be molecularly different from those of fertile women during the implantation window.