Human cell lines engineered for tetracycline‐regulated expression of tumor suppressor candidate genes from a frequently affected chromosomal region, 3p21

Abstract

We modified a tetracycline‐regulated system that can control the activity of individual genes quantitatively and reversibly in transgenic mammals. Despite these advances, there remained one problem in the intensive use of the tet‐system: the limited range of acceptor cell lines, expressing a tetracycline‐controlled transcriptional activator (tTA). This study describes in detail new vectors and a unifying strategy to generate tTA‐expressing cell lines.