Integrating Optoelectronic Tweezers for Individual Particle Manipulation with Digital Microfluidics Using Electrowetting-On-Dielectric (EWOD)

G. J. Shah, P. Chiou, J. Gong, A. Ohta, J. B. Chou, Ming C. Wu, C. Kim
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引用次数: 13

Abstract

This paper presents the integration of two powerful technologies: manipulation of droplets (i.e., digital microfluidics) using electrowettingon-dielectric (EWOD) and manipulation of individual particle inside the droplets using optoelectronic tweezers (OET). A novel platform for maintaining a viable cell culture environment is proposed as an application example, in which EWOD operations bring droplets containing cells, medium and waste into and out of the cell environment and OET operations address and manipulate the individual cells in coordination with the fluidic operations. Functions of EWOD and OET required to realize the concept are demonstrated.
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利用介电润湿技术(EWOD)集成用于单个粒子操作的光电镊子与数字微流体
本文介绍了两种强大技术的集成:使用电润湿介质(EWOD)操纵液滴(即数字微流体)和使用光电镊子(OET)操纵液滴内的单个粒子。作为应用实例,提出了一种新的维持细胞培养环境的平台,其中EWOD操作将含有细胞、培养基和废物的液滴带入细胞环境,OET操作与流体操作协同处理和操纵单个细胞。演示了实现该概念所需的EWOD和OET功能。
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