[The effect of the physiological state of Escherichia coli on the nucleoid ultrastructure under stress exposures].

A G Tkachenko, A A Chudinov, N S Churilova
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Abstract

The energetic state and activity of polyamine-synthesizing system of E. coli were studied under conditions of aerobic-anaerobic and pH transitions, nutrition shifts, osmotic and heat shocks. The electron microscopy of cells showed a correlation between cell physiological state and nucleoid ultrastructure. The role of energetic status and polyamines in the regulation of DNA supercoiling is discussed which is a putative cause for the changes observed in the nucleoid ultrastructure.

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[应激条件下大肠杆菌生理状态对类核超微结构的影响]。
研究了大肠杆菌在好氧-厌氧、pH转换、营养变化、渗透和热冲击等条件下多胺合成系统的能态和活性。细胞电镜显示细胞生理状态与类核超微结构之间存在相关性。讨论了能量状态和多胺在DNA超卷曲调控中的作用,这是在类核超微结构中观察到的变化的一个假定原因。
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