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[Cyclic AMP in the kidneys of adrenalectomized and nonadrenalectomized normal rats and rats with reflex dystrophy against a background of organ denervation and a total block of the body's beta-adrenoreceptors by propranolol]. [肾上腺切除和非肾上腺切除的正常大鼠和反射性营养不良大鼠在器官去神经支配和心得安完全阻断身体β -肾上腺素受体的背景下肾脏中的环AMP]。
Ia I Azhipa, L K Egorova

Using the radioimmunological assay, renal cAMP content was studied in adrenalectomized and non-adrenalectomized rats with reflex dystrophy resulting from chronic ischiatic nerve stimulation against the background either of organ denervation or of general beta-adreno-blockade with propranolol. It was shown that the surgical denervation of kidney resulted in an increase in renal cAMP both in norm and especially in reflex dystrophy. The beta-adrenoceptor blockade with propranolol was accompanied by a decrease in renal nucleotide being more pronounced in animals with intact ischiatic nerve. Based on own and literature data we have suggested that one cause for the damage to renal aldosterone receptors in reflex dystrophy under pathological stimulation from the focus of damage in ischiatic nerve as well as for their improved functioning in surgical or pharmacological (beta-adrenoceptor blockade) desympathization which discontinues this stimulation, may be change in cAMP content. Via cAMP-dependent protein kinases, this nucleotide triggers a complex system of intracellular metabolic transformations which is able to change the protein spectrum of cytoplasm, nucleus, and chromatin and, therefore, the protein complex of aldosterone receptors. A participation of other biochemical systems of aldosterone stimulus transmission, including cAMP-independent ones, in processes of damage and normalization of tubule cells of the kidneys undergoing reflex dystrophy cannot be excluded.

用放射免疫法研究了慢性坐骨神经刺激引起的反射性营养不良大鼠肾cAMP的含量,这些大鼠是由器官去神经支配或普萘洛尔阻断-肾上腺素引起的。结果表明,在正常情况下,尤其是在反射性营养不良的情况下,肾脏手术去神经支配导致肾cAMP升高。在坐骨神经完整的动物中,用心得安阻断β -肾上腺素能受体可伴有肾核苷酸的减少。基于自身和文献资料,我们认为坐骨神经损伤的焦点在病理刺激下对反射性营养不良的肾醛固酮受体的损害,以及它们在手术或药物(β -肾上腺素能受体阻断)失交感中功能的改善,可能是cAMP含量的改变。通过camp依赖性蛋白激酶,该核苷酸触发细胞内代谢转化的复杂系统,该系统能够改变细胞质、细胞核和染色质的蛋白质谱,从而改变醛固酮受体的蛋白质复合物。不能排除醛固酮刺激传递的其他生化系统,包括不依赖camp的生化系统,参与了肾小管细胞发生反射性营养不良的损伤和正常化过程。
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引用次数: 0
[Low-molecular heparin complex formation with the blood coagulation proteins--thrombin and fibrinogen]. [低分子肝素复合物与凝血蛋白——凝血酶和纤维蛋白原的形成]。
B A Kudriashov, L A Liapina, V E Pastorova, M V Kondashevskaia

It has been found that low molecular heparin (LMH) forms complexes with fibrinogen and thrombin. The formation of the heparin-fibrinogen and heparin-thrombin complexes has been testified by cross-linked electrophoresis. The reaction of complex formation was carried out at variable weight ratios of the components, i.e. 1:3 and 1:6, respectively. This complex causes lysis of unstabilized clots of fibrin. All these complexes manifested a slight anticoagulative activity.

研究发现,低分子肝素与纤维蛋白原和凝血酶形成复合物。交联电泳证实了肝素-纤维蛋白原和肝素-凝血酶复合物的形成。在不同组分的质量比(分别为1:3和1:6)下进行络合物生成反应。这种复合物导致不稳定的纤维蛋白凝块的溶解。这些复合物均表现出轻微的抗凝活性。
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引用次数: 0
[Adaptation to chemical mutagens]. [对化学诱变剂的适应]。
I V Serebriakova, M M Fomina, G G Poroshenko

Pre-adaptation of normal and tumor cells to the action of methylnitrosourea was studied. To attain these ends, a single low dose (10 mg/kg) was administered to animals two hours prior to the administration of main therapeutic dose (100 mg/kg) and the number of chromosomal rearrangements was determined. A significant decrease in translocation incidence was observed in metaphases of Ehrlich-ICP ascitic strain. Similar results were obtained on cells of murine bone marrow. The mechanisms of pre-adaptation to the action is discussed.

研究了正常细胞和肿瘤细胞对甲基亚硝基脲作用的预适应。为了达到这些目的,在给予主治疗剂量(100 mg/kg)前2小时给予动物单次低剂量(10 mg/kg),并测定染色体重排的数量。在Ehrlich-ICP腹水毒株的中期,易位发生率显著降低。在小鼠骨髓细胞上也得到了类似的结果。讨论了对动作的预适应机制。
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引用次数: 0
[The effect of therapy with cytostatics and immunomodulators on mitotic pathology in Lewis tumor cells in mice]. [细胞抑制剂和免疫调节剂对小鼠Lewis肿瘤细胞有丝分裂病理的影响]。
Iu N Potapov, T V Krutova, V S Pashkova, D V Khaleev

The effect of cytostatics (methylnitrosourea and methotrexate), immunomodulators (thymalin and reaferon) and their combinations on the mitotic pathology of mice Lewis tumour cells was studied. It was revealed that chemotherapy with these agents changed the interrelation between mitotic phases and somewhat enhanced the incidence of pathologic mitoses mainly connected with the damage of mitotic apparatus. Immunomodulators differently affected the cytostatic activity of antitumour agents that may be associated with their mechanisms of action.

研究了细胞抑制剂(甲基亚硝基脲和甲氨蝶呤)、免疫调节剂(胸腺素和干扰素)及其联合用药对小鼠Lewis肿瘤细胞有丝分裂病理的影响。结果表明,这些药物的化疗改变了有丝分裂期的相互关系,并在一定程度上增加了主要与有丝分裂器损伤有关的病理性有丝分裂的发生率。免疫调节剂不同程度地影响抗肿瘤药物的细胞抑制活性,这可能与其作用机制有关。
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引用次数: 0
[The qualitative metabolic characteristics of antitumor compounds]. [抗肿瘤化合物的定性代谢特征]。
S V Geodakian, V A Chernov
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引用次数: 0
[The quantitative assessment of the biological activity of angiogenin]. 【血管生成素生物活性的定量评价】。
G S Komolova, A D Mashtakova, A A Klesov

Methods have been developed for the in vivo quantitative estimation of biological activity of angiogenin, a protein growth factor of blood vessels. The quantitative analysis of angiogenin with the methods of angiogenetic disks in isotopic modification requires nanogram amounts of the angiogenetic. The cutireaction test is less sensitive though technically simple. The methods are developed on animal (mouse and rat) tissues.

血管生成素是一种血管生长蛋白因子,研究了血管生成素体内生物活性的定量测定方法。在同位素修饰血管生成盘的方法中,血管生成素的定量分析需要纳克量的血管生成素。皮肤反应测试虽然技术上简单,但灵敏度较低。这些方法是在动物(小鼠和大鼠)组织上发展起来的。
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引用次数: 0
[Changes in the intensity of protein synthesis in the gastric mucosa following the laser irradiation of a duodenal ulcer]. [激光照射十二指肠溃疡后胃黏膜蛋白质合成强度的变化]。
V Ia Brodskiĭ, S I Rapoport, V I Fateeva, S L Zaguskin, M I Rasulov, N V Nechaeva, N K Malinovskaia

The intensity of protein synthesis (incorporation/pool) is usually low in normal stomach mucosa and enhanced in exacerbation of peptic ulcer; following the effective laser therapy it decreases only in one third of cases. Therefore the scarring of ulcer not always coincides with the normalization of protein synthesis. The results characterize also the community of reactions in stomach and duodenal mucose membranes. The changes in protein synthesis intensity following the laser irradiation depended neither on the initial size of ulcer nor on the age of patient and the total duration of disease.

正常胃粘膜的蛋白质合成强度(掺入/池)通常较低,在消化性溃疡加重时增强;经过有效的激光治疗后,只有三分之一的病例会减少。因此,溃疡的瘢痕形成并不总是与蛋白质合成的正常化一致。结果还表征了胃和十二指肠粘膜的反应群落。激光照射后蛋白质合成强度的变化既不取决于溃疡的初始大小,也不取决于患者的年龄和疾病的总持续时间。
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引用次数: 0
[The factors influencing the sound activity of forest voles]. [影响森林田鼠声音活动的因素]。
M V Rutovskaia

The article is devoted to the effect of context on the acoustic activity of bank voles of 4 species (Clethrionomys glareolus, C. centralis, C. rutilus, and C. rufocanus) living in experimental groups. In addition to species differences and social state of a specimen in the group, the acoustic activities of vole is affected by such factors as locomotor activity of the vole itself and its partners (especially dominant ones) and aggression of surrounding voles.

本文研究了环境对4种田鼠(Clethrionomys glareolus, C. centralis, C. rutilus和C. rufocanus)听觉活动的影响。田鼠的声学活动除了受到种群内物种差异和种群状态的影响外,还受到自身及其同伴(尤其是优势体)的运动活动和周围田鼠的攻击性等因素的影响。
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引用次数: 0
[Hyperautophagy: the pathogenic characteristics of the cells in the Chediak-Higashi syndrome]. 【超自噬:Chediak-Higashi综合征细胞的致病特性】。
V V Rogovin, R A Murav'ev, V A Fomina
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引用次数: 0
[The pattern of the expression of homeo box-containing genes in the mesenchyme of the mammalian limb bud as a coordinate network for subsequent morphogenetic processes]. [哺乳动物肢芽间质中含有同源盒的基因作为后续形态发生过程的协调网络的表达模式]。
A V Spirov

Hypothesis is developed about mechanisms of determination of limb bud of mammals as a result of expression of homeo box containing genes (hox genes) of known up to date four hox families. Spatial pattern of the expression plays a role of coordinate system for subsequent processes of morphogenesis and differentiation of the limbs. Propositions of the hypothesis are realized in a computer dynamic model. The results of modeling are compared with known data on features of the expression of hox genes in the mesenchyme of developing limb buds in mammals and birds.

根据目前已知的4个hox家族同源盒基因(hox基因)的表达,提出了哺乳动物肢芽决定机制的假说。表达的空间格局对肢体的形态发生和分化的后续过程起着坐标系统的作用。该假设的命题在计算机动态模型中实现。将模拟结果与已知的哺乳动物和鸟类肢芽发育间质中hox基因表达特征的数据进行了比较。
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引用次数: 0
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